Belz Gabrielle T
The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.
Methods Mol Biol. 2010;595:351-69. doi: 10.1007/978-1-60761-421-0_23.
Dendritic cells (DCs) are a heterogeneous population of professional antigen-presenting cells (APCs) that play a major role in the initiation of immune responses. DC subsets differ in their anatomical locations together with their intrinsic abilities to capture, process, and present antigens on their major histocompatibility (MHC) class I and class II molecules. These features enable each DC subset to have distinct roles in immunity to infection and in the maintenance of self-tolerance. The discrete features of DC subpopulations have largely been defined by cell surface phenotype and anatomical location, rather than function. We have developed direct ex vivo methods to efficiently isolate small numbers of DCs from lymph node (LN) draining tissues and infectious sites to allow fine probing of their function using very sensitive antigen-specific LacZ hybridomas and in vitro proliferation of CFSE-labeled T cells. These approaches are particularly sensitive for detecting endogenous antigens derived from pathogens and self-tissues. Understanding these interactions has begun to allow us to understand how integration of different populations in the DC network responds to multiple scenarios of infection.
树突状细胞（DCs）是一类异质性的专职抗原呈递细胞（APCs），在免疫反应的启动中起主要作用。DC亚群在其解剖位置以及捕获、处理抗原并将其呈递于主要组织相容性复合体（MHC）I类和II类分子上的内在能力方面存在差异。这些特性使每个DC亚群在抗感染免疫和维持自身耐受性方面具有不同的作用。DC亚群的离散特征很大程度上是由细胞表面表型和解剖位置而非功能所定义的。我们已经开发出直接的体外方法，可从引流淋巴结（LN）的组织和感染部位有效分离少量DC，以便使用非常敏感的抗原特异性LacZ杂交瘤和CFSE标记的T细胞进行体外增殖，从而精细探究其功能。这些方法对于检测源自病原体和自身组织的内源性抗原特别敏感。对这些相互作用的理解已开始使我们明白DC网络中不同群体的整合如何应对多种感染情况。
