Flesher J W, Myers S R
Department of Pharmacology, A. B. Chandler Medical Center, University of Kentucky, Lexington 40536.
Life Sci. 1991;48(8):843-50. doi: 10.1016/0024-3205(91)90100-p.
The metabolism of benzene and toluene was investigated in preparations of human bone marrow incubated with S-adenosyl-L-methionine. Benzene undergoes a methyl-substitution reaction to yield toluene as a metabolite. Furthermore, toluene undergoes methyl-substitution in preparations of human bone marrow incubated with S-adenosyl-L-methionine to yield o-xylene, m-xylene, and p-xylene. Metabolites were detected by gas chromatography and mass spectroscopy. No metabolism of either benzene or toluene was detected when a boiled bone marrow preparation was used in the incubation, demonstrating the enzymatic nature of the S-adenosyl-L-methionine dependent methylation of both benzene and toluene.
在与S-腺苷-L-甲硫氨酸一起孵育的人骨髓制剂中研究了苯和甲苯的代谢。苯发生甲基取代反应，产生甲苯作为代谢产物。此外，在与S-腺苷-L-甲硫氨酸一起孵育的人骨髓制剂中，甲苯发生甲基取代，生成邻二甲苯、间二甲苯和对二甲苯。代谢产物通过气相色谱和质谱检测。当在孵育中使用煮沸的骨髓制剂时，未检测到苯或甲苯的代谢，这表明苯和甲苯的S-腺苷-L-甲硫氨酸依赖性甲基化具有酶促性质。
