Moe Kyaw Kyaw, Yano Takahisa, Kuwano Atsutoshi, Sasaki Satomi, Misawa Naoaki
Laboratory of Veterinary Public Health, Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, USA.
J Vet Med Sci. 2010 Feb;72(2):235-9. doi: 10.1292/jvms.09-0404. Epub 2009 Nov 27.
Equine canker is a chronic pododermatitis of the hoof in horses. Although spirochetes are detectable histopathologically in the lesions, the precise etiology remains unclear. This study reports the 16S rRNA gene sequencing of randomly selected clones based on PCR with Treponema-specific primers, using the canker lesions from two horses and healthy frog and sole from a horse. A total of 114 clones were obtained from the lesions, but no clones were detected in the healthy hoof tissues. The clones from the canker lesions examined were grouped into 19 operational taxonomic units, such as treponemal phylotypes originating from papillomatous digital dermatitis lesions of dairy cattle and as-yet uncultured human oral treponemes, indicating the presence of multiple treponemes in the lesions.
马溃疡性淋巴管炎是马蹄的一种慢性足皮炎。尽管在病变组织的组织病理学检查中可检测到螺旋体，但确切病因仍不清楚。本研究报告了基于PCR和密螺旋体特异性引物对随机选择的克隆进行16S rRNA基因测序的结果，使用了两匹马的溃疡性淋巴管炎病变组织以及一匹马的健康蹄叉和蹄底组织。从病变组织中共获得114个克隆，但在健康蹄组织中未检测到克隆。对溃疡性淋巴管炎病变组织的克隆进行分组，分为19个操作分类单元，例如源自奶牛乳头瘤性皮炎病变的密螺旋体系统型以及尚未培养的人类口腔密螺旋体，这表明病变组织中存在多种密螺旋体。
