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用于透射电子显微镜检查的石蜡包埋活检组织再处理方法的价值。

The value of the reprocessing method of paraffin-embedded biopsies for transmission electron microscopy.

作者信息

Lighezan Rodica, Baderca Flavia, Alexa Aurora, Iacovliev M, Bonţe Diana, Murărescu Elena Doina, Nebunu Adriana

机构信息

Department of Histology, "Victor Babes" University of Medicine and Pharmacy, Timisoara, Romania.

出版信息

Rom J Morphol Embryol. 2009;50(4):613-7.

Abstract

Transmission electron microscopy (TEM) implies an elaborate preparation protocol that includes: fixation in glutaraldehyde followed by osmium tetraoxide postfixation, specimen dehydration, infiltration, resin embedding, ultrathin sectioning and staining with heavy metal salts. The aim of TEM is to examine the ultrastructure of specimens in ways that cannot be examined using other equipments or techniques. In some cases, when the requirement for TEM were made after tissue collection, useful information can be obtained from reprocessing the formalin-fixed, wax-embedded tissue used for light microscopy.

摘要

透射电子显微镜(TEM)需要一套复杂的制备方案,包括:先用戊二醛固定,然后用四氧化锇进行后固定,对标本进行脱水、渗透、树脂包埋、超薄切片以及用重金属盐染色。TEM的目的是以其他设备或技术无法实现的方式检查标本的超微结构。在某些情况下,如果在组织采集后才提出进行TEM检查的要求,那么通过对用于光学显微镜检查的福尔马林固定、石蜡包埋组织进行重新处理,也可以获得有用的信息。

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