Chemical Engineering Laboratory, Faculty of Sciences, University of La Coruña, Rua Alejandro de la Sota, 1, E-15008, La Coruña, Spain.
Bioresour Technol. 2010 Apr;101(8):2608-15. doi: 10.1016/j.biortech.2009.10.060. Epub 2009 Nov 26.
Gas-phase styrene removal using the fungus, Sporothrix variecibatus was evaluated in a novel monolith bioreactor, receiving a continuous supply of nutrients from the trickling liquid phase. During the start-up process, the monolith reactor was operated for 22 days with relatively low styrene concentrations in the gas-phase (<0.4 g m(-3)). Afterwards, continuous experiments were carried out at different inlet styrene concentrations, ranging between 0.06 and 2.5 g m(-3), and at two different flow rates corresponding to empty bed residence times (EBRTs) of 77 and 19 s, respectively. A maximum elimination capacity of 67.4 g m(-3) h(-1) was observed at an inlet styrene load of 73.5 g m(-3) h(-1). However, it was observed that the critical loading rates to the monolith bioreactor were a strong function of the gas residence time. The critical load, with greater than 95% styrene removal was 74 g m(-3) h(-1) at an EBRT of 77 s, while it was only 37.2 g m(-3) h(-1) for an EBRT of 19 s. After 92 days of continuous operation, due to excess biomass growth on the surface of the monolith, the biodegradation efficiency decreased significantly. To ascertain the instantaneous response of the attached fungus, to withstand fluctuations in loading conditions, two dynamic shock loads were conducted, at EBRTs of 77 and 19 s, respectively. It was observed that, the performance of the monolith bioreactor decreased significantly at low residence times, when subjected to high shock loads. The recovery times for high performance, in both cases, did not exceed more than 1 h.
采用新型整体式生物反应器，利用枝顶孢（Sporothrix variecibatus）在气相中去除苯乙烯。在启动过程中，整体式生物反应器在气相中较低的苯乙烯浓度（<0.4 g m(-3))下运行了 22 天。随后，在不同的入口苯乙烯浓度下进行了连续实验，范围在 0.06 到 2.5 g m(-3)之间，并且在两个不同的空床停留时间（EBRT）下进行了实验，分别为 77 和 19 s。在入口苯乙烯负荷为 73.5 g m(-3) h(-1)时，观察到最大消除容量为 67.4 g m(-3) h(-1)。然而，观察到，整体式生物反应器的临界负荷率强烈依赖于气体停留时间。在 EBRT 为 77 s 时，临界负荷为 74 g m(-3) h(-1)，苯乙烯去除率大于 95%，而在 EBRT 为 19 s 时，临界负荷仅为 37.2 g m(-3) h(-1)。经过 92 天的连续运行，由于生物量在整体式生物反应器表面的过度生长，生物降解效率显著下降。为了确定附着真菌对负荷条件波动的瞬时响应，在 EBRT 分别为 77 和 19 s 时进行了两次动态冲击负荷实验。结果表明，在低停留时间下，当受到高冲击负荷时，整体式生物反应器的性能显著下降。在这两种情况下，恢复高性能的时间都不超过 1 小时。
