Post-graduate Program in Pharmaceutical Sciences, Pharmacy Faculty, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Toxicon. 2010 Apr 1;55(4):795-804. doi: 10.1016/j.toxicon.2009.11.013. Epub 2009 Nov 26.
Some proteins present in snake venom possess enzymatic activities, such as phospholipase A(2) and l-amino acid oxidase. In this study, we verify the action of the Bothrops marajoensis venom (BmarTV), PLA(2) (BmarPLA(2)) and LAAO (BmarLAAO) on strains of bacteria, yeast, and Leishmania sp. The BmarTV was isolated by Protein Pack 5PW, and several fractions were obtained. Reverse phase HPLC showed that BmarPLA(2) was isolated from the venom, and N-terminal amino acid sequencing of sPLA(2) showed high amino acid identity with other lysine K49 sPLA(2)s isolated from Bothrops snakes. The BmarLAAO was purified to high molecular homogeneity and its N-terminal amino acid sequence demonstrated a high degree of amino acid conservation with others LAAOs. BmarLAAO was able to inhibit the growth of P. aeruginosa, C. albicans and S. aureus in a dose-dependent manner. The inhibitory effect was more significant on S. aureus, with a MIC=50 microg/mL and MLC=200 microg/mL. However, the BmarTV and BmarPLA(2) did not demonstrate inhibitory capacity. BmarLAAO was able to inhibit the growth of promastigote forms of L. chagasi and L. amazonensis, with an IC(50)=2.55 microg/mL and 2.86 microg/mL for L. amazonensis and L. chagasi, respectively. BmarTV also provided significant inhibition of parasitic growth, with an IC(50) of 86.56 microg/mL for L. amazonensis and 79.02 microg/mL for L. chagasi. BmarPLA(2) did not promote any inhibition of the growth of these parasites. The BmarLAAO and BmarTV presented low toxicity at the concentrations studied. In conclusion, whole venom as well as the l-amino acid oxidase from Bothrops marajoensis was able to inhibit the growth of several microorganisms, including S. aureus, Candida albicans, Pseudomonas aeruginosa, and Leishmania sp.
一些存在于蛇毒液中的蛋白质具有酶活性，如磷脂酶 A(2)和 L-氨基酸氧化酶。在这项研究中，我们验证了马雅若蛇毒液（BmarTV）、PLA(2)（BmarPLA(2)）和 LAAO（BmarLAAO）对细菌、酵母和 Leishmania sp. 菌株的作用。BmarTV 是通过 Protein Pack 5PW 分离的，得到了几个馏分。反相 HPLC 显示 BmarPLA(2)是从毒液中分离出来的，并且 sPLA(2)的 N 端氨基酸序列与从 Bothrops 蛇中分离出的其他赖氨酸 K49 sPLA(2)具有很高的氨基酸同源性。BmarLAAO 被纯化到高分子均一性，其 N 端氨基酸序列与其他 LAAOs 具有高度的氨基酸保守性。BmarLAAO 能够以剂量依赖的方式抑制铜绿假单胞菌、白色念珠菌和金黄色葡萄球菌的生长。对金黄色葡萄球菌的抑制作用更为显著，MIC=50μg/mL，MLC=200μg/mL。然而，BmarTV 和 BmarPLA(2) 没有表现出抑制能力。BmarLAAO 能够抑制 L. chagasi 和 L. amazonensis 前鞭毛体的生长，IC(50)分别为 2.55μg/mL 和 2.86μg/mL。BmarTV 也对寄生虫的生长有显著的抑制作用，对 L. amazonensis 和 L. chagasi 的 IC(50)分别为 86.56μg/mL 和 79.02μg/mL。BmarPLA(2) 没有促进这些寄生虫的任何生长抑制。BmarLAAO 和 BmarTV 在研究浓度下表现出低毒性。总之，马雅若蛇毒液以及 L-氨基酸氧化酶能够抑制几种微生物的生长，包括金黄色葡萄球菌、白色念珠菌、铜绿假单胞菌和 Leishmania sp.。
