Electron Microscope Unit, University of Cape Town, 7701 Cape Town, South Africa.
Ultramicroscopy. 2010 Jan;110(2):170-5. doi: 10.1016/j.ultramic.2009.11.001. Epub 2009 Nov 18.
The scanning electron microscope produces nanometer-resolution surface images of biological samples preserved in a life-like state. Extracting three-dimensional information from these two-dimensional images has been the subject of long and ongoing research. We present here a general method and theoretical basis for reconstructing the surfaces of SEM specimens imaged from multiple directions by back-projection. The resulting reconstructions are faithful representations of the original specimen geometry, even when the input images are blurred and have low signal-to-noise ratio.
扫描电子显微镜可生成以生活状态保存的生物样本的纳米分辨率表面图像。从这些二维图像中提取三维信息一直是长期持续研究的课题。我们在此介绍了一种通过反向投影从多个方向对 SEM 样本成像进行重建的通用方法和理论基础。即使输入图像模糊且信噪比较低，重建结果也是原始样本几何形状的忠实表示。
