胶原消化对大鼠膈肌被动弹性特性的影响。
Effect of collagen digestion on the passive elastic properties of diaphragm muscle in rat.
机构信息
Biomechanics Laboratory, Division of Orthopedic Research, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
出版信息
Med Eng Phys. 2010 Jan;32(1):90-4. doi: 10.1016/j.medengphy.2009.11.002. Epub 2009 Nov 27.
Effects of collagen digestion have been defined up to the fibril level. However, the question remains as to whether the alteration of skeletal muscle extracellular matrix (ECM) affects a muscle's passive elastic response. Various elastography methods have been applied as tools for evaluating the mechanical properties and ECM content of skeletal muscle. In an effort to develop an ECM altered skeletal muscle model, this study determined the effect of collagen digestion on the passive elastic properties of skeletal muscle. Passive mechanical properties of rat diaphragms were evaluated in various degrees of collagen digestion. Between cyclic loading tests, muscle strips were immersed in various concentrations of clostridium histolyticum derived bacterial collagenase. All samples were later viewed via light microscopy. Cyclic testing revealed linear relationships between passive muscle stiffness and digestion time at multiple concentrations. These results demonstrate that collagenase digestion of the ECM in skeletal muscle could be used as a simple and reliable model of mechanically altered in vitro tissue samples.
胶原消化的影响已被定义到原纤维水平。然而，仍然存在一个问题，即骨骼肌细胞外基质（ECM）的改变是否会影响肌肉的被动弹性反应。各种弹性成像方法已被用作评估骨骼肌机械性能和 ECM 含量的工具。为了开发一种 ECM 改变的骨骼肌模型，本研究旨在确定胶原消化对骨骼肌被动弹性特性的影响。在不同程度的胶原消化过程中，评估了大鼠横膈膜的被动机械性能。在周期性加载试验之间，将肌肉条浸泡在不同浓度的来源于梭菌组织蛋白酶的细菌胶原酶中。所有样本随后通过光学显微镜进行观察。周期性测试表明，在多个浓度下，被动肌肉硬度与消化时间之间存在线性关系。这些结果表明，胶原酶消化骨骼肌中的 ECM 可以作为一种简单可靠的体外组织样本机械改变模型。
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