Department of Chemistry, MITE, Moodabidri, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Jan;75(1):14-20. doi: 10.1016/j.saa.2009.08.032. Epub 2009 Aug 19.
The interactions of Acridine Orange with Sodium Alginate and Pinacyanol Chloride with Heparin have been investigated by spectrophotometric method. The polymers induce metachromasy in the dye as evidenced from the considerable blue shift in the absorption maxima of the corresponding dyes. The interaction constant and thermodynamic parameters of polymer-dye interactions have been determined. The effect of additives such as alcohols, and urea on the reversal of metachromasy has been studied. The data has been used to determine the stability of the metachromatic complex and the nature of binding. The thermodynamic parameters of interaction revealed that binding between Acridine Orange and Sodium Alginate involved only electrostatic forces while that between Pinacyanol Chloride involved both electrostatic and hydrophobic forces. The reversal studies using surfactants indicated the involvement of both electrostatic and hydrophobic forces in binding. Based on the results it can be concluded that Pinacyanol Chloride is more effective inducing metachromasy than Acridine Orange.
吖啶橙与海藻酸钠以及派洛宁氯与肝素的相互作用已通过分光光度法进行了研究。聚合物使染料发生变色反应，这可从相应染料的吸收最大值发生相当大的蓝移得到证明。已经确定了聚合物-染料相互作用的相互作用常数和热力学参数。研究了添加剂（例如醇和尿素）对变色反应逆转的影响。已使用该数据来确定变色配合物的稳定性和结合的性质。相互作用的热力学参数表明，吖啶橙与海藻酸钠之间的结合仅涉及静电力，而派洛宁氯之间的结合涉及静电力和疏水力。使用表面活性剂进行的逆转研究表明，结合涉及静电力和疏水力。根据结果可以得出结论，派洛宁氯比吖啶橙更有效地诱导变色反应。
