DNA Chip Research Inc, Suehirocho, Tsurumi-ku, Yokohama 230-0045, Japan.
Biochem Biophys Res Commun. 2010 Jan 1;391(1):846-51. doi: 10.1016/j.bbrc.2009.11.150. Epub 2009 Nov 27.
A 4-h bout of exercise induces immunomodulatory effects. Peripheral blood was withdrawn before, and at 4, 8 and 24h after the start of exercise. RNA from the unfractionated white blood cells was analyzed using Agilent human 44K microarray. The expression profiles were sorted into seven clusters based on their unique time-dependent kinetics. In a separate experiment, cell-specific markers were collected and compared among the members in each cluster. Two clusters were assigned as representing neutrophils, one as NK cells, and another mostly as T cells. Three clusters seemed to be mixtures of several cell types. Extension of this approach to other systems is discussed.
一次 4 小时的运动即可诱导免疫调节效应。在运动开始前、开始后 4、8 和 24 小时时抽取外周血。使用 Agilent 人类 44K 微阵列分析未分馏的白细胞中的 RNA。根据其独特的时间依赖性动力学，将表达谱分为七个簇。在一个单独的实验中，收集细胞特异性标志物并在每个簇中的成员之间进行比较。两个簇被指定为代表中性粒细胞，一个为 NK 细胞，另一个主要为 T 细胞。三个簇似乎是几种细胞类型的混合物。讨论了将这种方法扩展到其他系统的问题。
