Department of Pharmaceutical Biotechnology and Pharmaceutical Sciences Research Center, School of Pharmacy, Shiraz University of Medical Sciences, PO Box 71345-1583, Shiraz, Iran.
Bioresour Technol. 2010 Mar;101(6):2059-62. doi: 10.1016/j.biortech.2009.11.032. Epub 2009 Nov 28.
The production of biodiesel from a naturally isolated strain of Chlamydomonas was investigated. The microalgal strain was isolated from the rice paddy-field soil samples during a screening program. The identification was done using physiological and molecular approaches. After reaching the stationary phase of growth, the total content of the lipids was extracted. The extracted fatty acids were primarily esterified and then identified through TLC and GC/MS analysis. Several types of fatty acid methyl esters (FAMEs) were identified in the isolated microalga and the presence of at least nine FAMEs in Chlamydomonas sp. MCCS 026 was shown. The total fatty acid content of the isolated strain was 25%. The composition of fatty acids in the studied species of microalga was mainly docosanoic acid methyl ester, tetradecanoic acid methyl ester, hexadecanoic acid methyl ester and nonanoic acid methyl ester.
从一株自然分离的衣藻中生产生物柴油的研究。在筛选计划中，从稻田土壤样本中分离出微藻菌株。采用生理和分子方法进行鉴定。当达到生长的稳定期时，提取总脂质含量。提取的脂肪酸先酯化，然后通过 TLC 和 GC/MS 分析进行鉴定。在分离的微藻中鉴定出几种脂肪酸甲酯（FAME），并表明衣藻 sp. MCCS 026 中至少存在 9 种 FAME。分离菌株的总脂肪酸含量为 25%。研究的微藻物种中脂肪酸的组成主要为二十二酸甲酯、十四酸甲酯、十六酸甲酯和壬酸甲酯。
