酸不稳定的α干扰素和ω-1干扰素是否相同？
Are the acid-labile interferon alpha and interferon omega-1 identical?
作者信息
Kontsek P, Borecký L, Novák M
机构信息
Institute of Virology, Slovak Academy of Sciences, Bratislava.
出版信息
Virology. 1991 Mar;181(1):416-8. doi: 10.1016/0042-6822(91)90517-f.
The interferon (IFN) activity found in human leukocyte IFN alpha preparations, autoimmune and AIDS sera, and others was reported to have distinct antigenic and deviating biological properties. This led to its vague designation as acid-labile and thermolabile IFN alpha. However, using specific monoclonal antibodies, the acid-labile component of IFN alpha (not exposed to pH 2) and recombinant IFN omega 1 showed significant relatedness. Monoclonal antibody T19, generated with virus-induced leukocyte IFN alpha that had not been exposed to pH 2, neutralized both the antiviral and antiproliferative activities of IFN omega-1, and vice versa; monoclonal antibody OMG 5, specific for recombinant IFN omega-1, cross-neutralized the antiviral and antiproliferative effects of the acid-labile component of leukocyte IFN alpha. When these two IFN preparations were incubated at pH 2 for 72 hr, their biological activity significantly decreased.
据报道，在人白细胞干扰素α制剂、自身免疫性疾病和艾滋病患者血清以及其他样本中发现的干扰素（IFN）活性具有独特的抗原性和不同的生物学特性。这导致其被模糊地命名为酸不稳定和热不稳定的干扰素α。然而，使用特异性单克隆抗体，未暴露于pH 2的干扰素α的酸不稳定成分与重组干扰素ω1显示出显著的相关性。用未暴露于pH 2的病毒诱导白细胞干扰素α产生的单克隆抗体T19，可中和干扰素ω-1的抗病毒和抗增殖活性，反之亦然；针对重组干扰素ω-1的特异性单克隆抗体OMG 5可交叉中和白细胞干扰素α酸不稳定成分的抗病毒和抗增殖作用。当将这两种干扰素制剂在pH 2下孵育72小时时，它们的生物学活性显著降低。
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