Maguire N C, Drozd K, Luff R D, Kantor R R
Department of Laboratory Sciences, College of Allied Health Sciences, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Acta Cytol. 1991 Jan-Feb;35(1):94-9.
A mouse monoclonal antibody and a rabbit polyclonal antibody prepared against the placental form of the enzyme glutathione S-transferase (GST-pi) were used to immunohistochemically stain normal and neoplastic human uterine cervical tissues from 88 cases. Of 65 cases of preneoplastic squamous lesions and invasive carcinomas of the cervix, 94% stained with the monoclonal antibody and 100% with the polyclonal antibody. In the 23 benign tissues, staining of ectocervical squamous epithelium was generally not observed; however, areas of reserve-cell hyperplasia, immature squamous metaplasia and adjacent endocervical cells did show staining (68% with the monoclonal antibody and 95% with the polyclonal antibody). Many of the positive tissue types showed a variety of staining patterns and intensities. These findings do not support the concept that GST-pi staining can be used to distinguish preneoplastic lesions of the cervix from benign reactive or proliferative processes. These results are of interest in the investigation of cervical carcinogenesis since GST-pi may be involved in an early stage of neoplastic transformation of the cervical epithelium. The correlation of these findings with the results of human papillomavirus testing and DNA content analysis should be of interest in determining the relationship of this enzyme to cervical neoplasia.
使用针对胎盘形式的谷胱甘肽S-转移酶（GST-π）制备的小鼠单克隆抗体和兔多克隆抗体，对88例正常和肿瘤性人子宫颈组织进行免疫组织化学染色。在65例宫颈肿瘤前鳞状病变和浸润癌中，94%的病例用单克隆抗体染色呈阳性，100%的病例用多克隆抗体染色呈阳性。在23例良性组织中，通常未观察到宫颈外鳞状上皮染色；然而，储备细胞增生、未成熟鳞状化生区域及相邻的宫颈内膜细胞确实显示出染色（单克隆抗体染色阳性率为68%，多克隆抗体染色阳性率为95%）。许多阳性组织类型呈现出多种染色模式和强度。这些发现不支持GST-π染色可用于区分宫颈肿瘤前病变与良性反应性或增殖性病变这一概念。这些结果在宫颈癌发生机制的研究中具有重要意义，因为GST-π可能参与宫颈上皮肿瘤转化的早期阶段。这些发现与人类乳头瘤病毒检测结果和DNA含量分析结果之间的相关性，对于确定这种酶与宫颈肿瘤的关系可能具有重要意义。
