Satoh T, Nishida M, Oki A, Ichikawa Y, Tsunoda H, Kubo T
Department of Obstetrics and Gynecology, Ibaraki-Seinan Medical Center Hospital.
Nihon Sanka Fujinka Gakkai Zasshi. 1996 Jul;48(7):522-8.
The glutathione S-transferase (GST) pi has been studied in association with the mechanisms of multidrug resistance and as a marker for malignant tumors. In this study, specimens from 92 cases of cervical neoplasms and 10 cases of normal squamous epithelium adhering to myoma were stained immunohistochemically with a rabbit polyclonal antibody to GST-pi. In 6 cases of normal squamous epithelium, the intermediate layer was positively stained with the GST-pi antibody. In all 20 cases of dysplasia, the cells with koilocytotic atypia were stained positively. In all 10 cases of carcinoma in situ and all 16 cases of stage Ia squamous cell carcinoma, various intensities of GST-pi staining were demonstrated. Forty-six specimens of stage Ib or more squamous cell carcinoma were positive for GST-pi binding except only one case. In general, squamous cell carcinoma of the uterine cervix is resistant to chemotherapeutic agents. GST-pi is most frequently stained in cervical squamous cell carcinoma as compared with ovarian or endometrial carcinoma. In conclusion, these results suggest that GST-pi may be a marker for cervical squamous cell carcinoma.
谷胱甘肽S-转移酶（GST）π已被用于研究多药耐药机制，并作为恶性肿瘤的标志物。在本研究中，用兔抗GST-π多克隆抗体对92例宫颈肿瘤标本和10例附着于肌瘤的正常鳞状上皮标本进行免疫组织化学染色。在6例正常鳞状上皮中，中间层被GST-π抗体阳性染色。在所有20例发育异常中，具有挖空细胞异型性的细胞呈阳性染色。在所有10例原位癌和所有16例Ia期鳞状细胞癌中，均显示出不同强度的GST-π染色。除1例之外，46例Ib期或更晚期鳞状细胞癌标本的GST-π结合呈阳性。一般来说，子宫颈鳞状细胞癌对化疗药物耐药。与卵巢癌或子宫内膜癌相比，GST-π在宫颈鳞状细胞癌中最常呈阳性染色。总之，这些结果表明GST-π可能是宫颈鳞状细胞癌的一个标志物。
