植物线粒体 RNA 编辑之路：哈利法克斯篇章。

The path to RNA editing in plant mitochondria: the Halifax chapter.

机构信息

Department of Biochemistry and Molecular Biology, Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, 5850 College Street, Halifax, Nova Scotia, Canada.

出版信息

IUBMB Life. 2009 Dec;61(12):1114-7. doi: 10.1002/iub.270.

DOI:10.1002/iub.270

PMID:19946899

Abstract

A single C/T difference between gene and cDNA within the 5'-untranslated region of the wheat mtDNA-encoded cox2 (cytochrome oxidase subunit 2) sequence catalyzed the discovery of C-to-U RNA editing in plant mitochondria by a group at Dalhousie University in Halifax, Nova Scotia [Covello and Gray, (1989) Nature 341, 662-666]. Editing of codons specifying essential copper-binding ligands in the Cox2p Cu(A) site provided additional insights about the amino acid residues comprising this site, as well as illustrating the functional importance of editing in wheat mitochondria. Further investigations of RNA editing in plant mitochondria led to the subsequent discovery of a case of mitochondrion-to-nucleus gene (cox2) transfer in soybean, mediated by reverse transcription of edited cox2 mRNA.

摘要

小麦线粒体编码 cox2（细胞色素氧化酶亚基 2）序列 5'非翻译区基因和 cDNA 之间的单个 C/T 差异，通过新斯科舍省哈利法克斯达尔豪斯大学的一个小组发现了植物线粒体中的 C-to-U RNA 编辑[Covello 和 Gray，（1989）自然 341,662-666]。在 Cox2p Cu(A) 位点指定必需铜结合配体的密码子编辑提供了有关该位点组成的氨基酸残基的更多信息，并且说明了编辑在小麦线粒体中的功能重要性。对植物线粒体中 RNA 编辑的进一步研究导致随后发现了大豆中线粒体到细胞核基因（cox2）转移的情况，这是由编辑的 cox2 mRNA 的逆转录介导的。