Mahjoub A, El Gharbi Mohamed Salah, Mguis K, El Gazzah Mohamed, Brahim N B
Laboratory of Botanic of Inrat, Hedi Karray Street, 2049 Ariana, Tunisia.
Pak J Biol Sci. 2009 Jul 15;12(14):994-1003. doi: 10.3923/pjbs.2009.994.1003.
Thirteen Aegilops geniculata Roth (geniculate goat grass) accessions from collection of the North and Central Tunisian (Cap-Bon, Mogodses, Kroumiry and the Dorsal areas) were used to assess its genetic diversity by morphological and Random Amplified Polymorphic DNA (RAPD) data and to evaluate relationship between morphological and RAPD markers. Nineteen morphological traits were analyzed on all accessions using Principal Analysis Component (PCA) and clusters were constrained based on median joining distances. Nineteen arbitrary universal primers were used for the amplification of random DNA sequences and generated 212 bands ranging from 0.5 to 3 kb with 71.27% polymorphism across the 13 accessions. Both RAPD and morphological data classified accessions in two main groups. Both methods were used to compare how morphological traits and RAPD molecular markers described accessions relationship and showed a high degree of variation among analyzed accessions, indicating an important source of genetic diversity that can be used in future breeding programs. Morphological PCA traits and cluster indicated climatic stage. In fact, they grouped Ae. geniculata accessions according to genetic criteria such as earliness and high kernel yield. Comparison of morphological and molecular data using the Mantel test indicated a non significant correlation (r = -0.268). Nevertheless, RAPD and selected morphological characters appear as useful and complementary techniques for evaluation of genetic diversity in Ae. geniculata.
从突尼斯北部和中部（邦角、莫戈德塞、克鲁米里和背斜地区）收集的13份节节麦（弯穗山羊草）种质用于通过形态学和随机扩增多态性DNA（RAPD）数据评估其遗传多样性，并评估形态学和RAPD标记之间的关系。使用主成分分析（PCA）对所有种质的19个形态性状进行分析，并基于中位连接距离构建聚类。使用19个任意通用引物扩增随机DNA序列，在13份种质中产生了212条大小在0.5至3 kb之间的条带，多态性为71.27%。RAPD和形态学数据均将种质分为两个主要类群。两种方法都用于比较形态性状和RAPD分子标记如何描述种质间的关系，结果表明在所分析的种质中存在高度变异，这表明其是未来育种计划中可利用的重要遗传多样性来源。形态学PCA性状和聚类表明了气候阶段。事实上，它们根据早熟和高产等遗传标准对节节麦种质进行了分组。使用Mantel检验比较形态学和分子数据表明两者之间不存在显著相关性（r = -0.268）。然而，RAPD和选定的形态学特征似乎是评估节节麦遗传多样性的有用且互补的技术。
