Song Hui-Xing, Zhong Zhang-Cheng
School of Life Science, Qufu Normal University, Qufu, Shandong, China.
Ying Yong Sheng Tai Xue Bao. 2009 Aug;20(8):1857-62.
A total of 17 herbages belonging to 12 families were selected as the host plants of arbuscular mycorrhizal fungi (AMF). They were grown on calcareous soil and purple soil, respectively, and sampled on the 120th day after seedling emergence. The DNA of AMF in the herbages roots was extracted by CTAB method, the partial rDNA sequence encoding ribosomal 28S big unit was amplified with special primers U1/U2 for fungi, and the PCR products were analyzed with PAGE silver staining method. In calcareous soil, 29 AMF bands were found in the roots of the 17 host plant species, and each host plant was colonized by 8.29 AMF bands; while in purple soil, only twenty-four AMF bands were found, with 9.47 bands in each host plant. All the AMF bands included unique bands and common bands. Cluster analysis showed that the AMF colonization in host plant roots was family-specific, and affected by soil factors. The feasibility of applying AMF in ecological restoration of limestone area was also discussed in this paper.
共选取了12科的17种草本植物作为丛枝菌根真菌（AMF）的宿主植物。它们分别种植在石灰土和紫色土上，并在出苗后第120天进行采样。采用CTAB法提取草本植物根系中AMF的DNA，用真菌特异性引物U1/U2扩增编码核糖体28S大亚基的部分rDNA序列，并用PAGE银染法分析PCR产物。在石灰土中，17种宿主植物根系中共发现29条AMF条带，每种宿主植物被8.29条AMF条带定殖；而在紫色土中，仅发现24条AMF条带，每种宿主植物有9.47条。所有AMF条带包括独特条带和共有条带。聚类分析表明，宿主植物根系中的AMF定殖具有科特异性，并受土壤因素影响。本文还探讨了AMF在石灰岩地区生态恢复中应用的可行性。
