Zepeda Salvador, Nakatsubo Shunichi, Furukawa Yoshinori
Institute of Low Temperature Science, Hokkaido University, Kita-19, Nishi-8 Kita-ku Sapporo, Hokkaido 060-0819, Japan.
Rev Sci Instrum. 2009 Nov;80(11):115102. doi: 10.1063/1.3222739.
A crystal growth apparatus was designed and built to study the effect of growth modifiers, antifreeze proteins and antifreeze glycoproteins (AFGPs), on ice crystal growth kinetics and morphology. We used a capillary growth technique to obtain a single ice crystal with well-defined crystallographic orientation grown in AFGP solution. The basal plane was readily observed by rotation of the capillary. The main growth chamber is approximately a 0.8 ml cylindrical volume. A triple window arrangement was used to minimize temperature gradients and allow for up to 10 mm working distance objective lens. Temperature could be established to within +/-10 mK in as little as 3.5 min and controlled to within +/-2 mK after 15 min for at least 10 h. The small volume growth chamber and fast equilibration times were necessary for parabolic flight microgravity experiments. The apparatus was designed for use with inverted and side mount configurations.
设计并制造了一种晶体生长装置，用于研究生长调节剂、抗冻蛋白和抗冻糖蛋白（AFGP）对冰晶生长动力学和形态的影响。我们使用毛细管生长技术在AFGP溶液中获得具有明确晶体学取向的单个冰晶。通过旋转毛细管可以很容易地观察到基面。主生长室的体积约为0.8毫升，呈圆柱形。采用三窗口布置以最小化温度梯度，并允许使用工作距离高达10毫米的物镜。温度可在短短3.5分钟内稳定在±10 mK以内，并在15分钟后控制在±2 mK以内，持续至少10小时。对于抛物线飞行微重力实验，小体积生长室和快速平衡时间是必要的。该装置设计用于倒置和侧装配置。
