Department of Comparative Biomedical Sciences, Teramo, Italy.
Exp Parasitol. 2010 Apr;124(4):361-4. doi: 10.1016/j.exppara.2009.11.014. Epub 2009 Dec 3.
Larvae of Rhinoestrus spp. (Diptera, Oestridae) infect nasal and sinus cavities of horses, causing a nasal myiasis characterized by severe respiratory distress. Presently, the diagnosis of horse nasal botfly relies on the observation of clinical signs, on the post mortem retrieval of larvae or on molecular assays performed using pharyngeal swabs. The present study was carried out to characterize larval somatic proteins and salivary glands of Rhinoestrus spp. in a preliminary assessment towards the immunodiagnosis of equine rhinoestrosis. Out of the 212 necropsied horses 13 were positive for the presence of Rhinoestrus spp. larvae. The analysis of the sera from the infected animals by Western blotting assay showed the presence of a specific host humoral immune response against Rhinoestrus spp. larvae and proved that the salivary glands are the major immunogens in horse nasal botflies.
Rhinostrus 属幼虫（双翅目，纹皮蝇科）感染马的鼻腔和鼻窦，引起以严重呼吸困难为特征的鼻蝇蛆病。目前，马鼻蝇蛆病的诊断依赖于临床症状的观察、死后幼虫的检出或使用咽拭子进行的分子检测。本研究旨在对 Rhinostrus 属幼虫的体壁蛋白和唾液腺进行鉴定，以期对马 Rhinostrosis 进行免疫诊断。在对 212 例剖检马进行分析中，有 13 例被检马的 Rhinostrus 属幼虫呈阳性。对感染动物血清进行 Western blot 分析显示，宿主对 Rhinostrus 属幼虫存在特异性体液免疫反应，证实唾液腺是马鼻蝇蛆的主要免疫原。
