组蛋白乙酰转移酶 p300 是转录因子 REL 的共激活因子，在人弥漫性大 B 细胞淋巴瘤细胞系 RC-K8 中 C 端截短。
Histone acetyltransferase p300 is a coactivator for transcription factor REL and is C-terminally truncated in the human diffuse large B-cell lymphoma cell line RC-K8.
机构信息
Department of Biology, Boston University, Boston, MA 02215, USA.
出版信息
Cancer Lett. 2010 May 28;291(2):237-45. doi: 10.1016/j.canlet.2009.10.018. Epub 2009 Nov 30.
Abstract
Human c-Rel (REL) is a member of the NF-kappaB family of transcription factors. REL's normal physiological role is in the regulation of B-cell proliferation and survival. The REL gene is amplified in many human B-cell lymphomas and overexpression of REL can transform chicken lymphoid cells. In this report, histone acetyltransferase p300 enhanced REL-induced transactivation and interacted with REL both in vitro and in REL-transformed chicken spleen cells and the B-lymphoma cell line RC-K8, in which REL is constitutively active and required for proliferation. However, due to a deletion in the EP300 locus, only a C-terminally truncated form of p300 is expressed in RC-K8 cells. These results suggest a role for p300 in REL-mediated oncogenic activity in B lymphoma.
摘要
人源 c-Rel（REL）是 NF-κB 转录因子家族的成员。REL 的正常生理作用是调节 B 细胞的增殖和存活。在许多人类 B 细胞淋巴瘤中扩增 REL 基因，并且 REL 的过表达可以转化鸡淋巴细胞。在本报告中，组蛋白乙酰转移酶 p300 增强了 REL 诱导的反式激活，并在体外以及 REL 转化的鸡脾细胞和 B 淋巴瘤细胞系 RC-K8 中与 REL 相互作用，在 RC-K8 细胞中 REL 是组成性激活的并且是增殖所必需的。然而，由于 EP300 基因座的缺失，仅在 RC-K8 细胞中表达 C 末端截断形式的 p300。这些结果表明 p300 在 B 淋巴瘤中 REL 介导的致癌活性中起作用。
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2
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3
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4
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6
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