Erciyes University, Faculty of Sciences and Arts, Department of Biology, Kayseri, Turkey.
Plant Physiol Biochem. 2010 Feb-Mar;48(2-3):160-6. doi: 10.1016/j.plaphy.2009.11.001. Epub 2009 Nov 20.
A hydroponic experiment was carried out in a growth chamber to investigate the impact of Selenium (Se) levels on physiological and biochemical characteristics of a barley cultivar. Membrane lipid peroxidation (LPO), proline accumulation and antioxidant activities of some enzymes of barley seedlings under Se toxicity were investigated. Significant increase in thiobarbituric acid reactive substance (TBARS) content, and a stimulation of catalase (CAT, 1.11.1.6), ascorbate peroxidase (APX, 1.11.1.11), glutathione reductase (GR, 1.6.4.2), and glutathione S-transferase (GST, 2.5.1.18) activities were recorded in barley seedlings subjected to 2, 4, 8, 16 ppm Se. Superoxide dismutase (SOD, EC 1.15.1.1) activity was not altered significantly. Plant height and chlorophyll content of the seedlings were also affected significantly in a dose dependent manner by Se treatment. Considerable amount of proline accumulation was also observed in response to Se treatment. The results indicated that increases in the activities of the antioxidant enzymes were not sufficient to protect cell membrane against Se toxicity.
在生长室中进行了一项水培实验，以研究硒（Se）水平对大麦品种生理生化特性的影响。研究了 Se 毒性下大麦幼苗的膜脂过氧化（LPO）、脯氨酸积累和一些酶的抗氧化活性。结果表明，2、4、8、16ppm Se 处理组大麦幼苗的丙二醛（TBARS）含量显著增加，过氧化氢酶（CAT，1.11.1.6）、抗坏血酸过氧化物酶（APX，1.11.1.11）、谷胱甘肽还原酶（GR，1.6.4.2）和谷胱甘肽 S-转移酶（GST，2.5.1.18）活性均受到刺激。超氧化物歧化酶（SOD，EC 1.15.1.1）活性无明显变化。Se 处理还显著影响幼苗株高和叶绿素含量，呈剂量依赖性。脯氨酸积累也有明显增加。结果表明，抗氧化酶活性的增加不足以保护细胞膜免受 Se 毒性的影响。
