Center for Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.
J Photochem Photobiol B. 2010 Jan 21;98(1):35-9. doi: 10.1016/j.jphotobiol.2009.10.005. Epub 2009 Nov 4.
We describe spectral properties of novel fluorescence probe DyLight 594. Absorption and fluorescence spectra of this dye are in the region of Alexa 594 fluor spectra. The quantum yield of DyLight 594 in conjugated form to IgG is higher than corresponding quantum yield of Alexa 594 by about 50%. The new DyLight dye also shows slightly longer lifetime and photostability. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make this fluorophore attractive as a fluorescence probe in biochemical/biological studies involving fluorescence methods.
我们描述了新型荧光探针 DyLight 594 的光谱特性。该染料的吸收和荧光光谱位于 Alexa 594 荧光光谱区域内。DyLight 594 与 IgG 结合的量子产率比相应的 Alexa 594 高约 50%。新的 DyLight 染料还表现出稍长的荧光寿命和光稳定性。这些有利的性质和高各向异性值，以及双光子激发的高截面，使得这种荧光团在涉及荧光方法的生化/生物学研究中作为荧光探针具有吸引力。
