Department of Otorhinolaryngology, Medical University of Vienna, 1090 Vienna, Austria.
J Surg Oncol. 2010 Feb 1;101(2):127-30. doi: 10.1002/jso.21444.
This retrospective study was performed to evaluate the status of p53 in pleomorphic adenomas and carcinomas ex pleomorphic adenoma in the parotid gland. As loss or mutation of p53 can cause malignant transformation, the possible degeneration of pleomorphic adenomas to carcinomas ex pleomorhic adenoma was investigated by mutational analysis.
Twenty-five Patients including 14 patients with pleomorphic adenomas and 11 patients with carcinoma ex pleomorphic adenoma of the parotid gland were examined for p53 status. DNA was extracted out of paraffin-embedded tissue and PCR was performed for the coding exons 2-11. Denaturing gradient gel electrophoresis (DGGE) was carried out for mutational analysis and DNA sequencing was performed in case of a suspected mutation.
Fourteen pleomorphic adenomas and 11 carcinomas ex pleomorphic adenoma were screened for p53 status and potent mutations. Subsequent sequencing of the distinct exons showed no mutation.
We could not detect mutations of p53 neither in benign nor malignant parotid tumors and we therefore assume that p53 plays no role in the transformation from pleomorphic adenoma to carcinoma ex pleomorphic adenoma.
本回顾性研究旨在评估腮腺多形性腺瘤和癌在多形性腺瘤中的 p53 状态。由于 p53 的缺失或突变可能导致恶性转化，因此通过突变分析研究了多形性腺瘤向癌在多形性腺瘤中的可能退行性变。
对 25 例患者进行了研究，包括 14 例多形性腺瘤和 11 例腮腺癌在多形性腺瘤患者，检测 p53 状态。从石蜡包埋组织中提取 DNA，并进行编码外显子 2-11 的 PCR。进行变性梯度凝胶电泳 (DGGE) 进行突变分析，在疑似突变的情况下进行 DNA 测序。
对 14 例多形性腺瘤和 11 例癌在多形性腺瘤患者进行了 p53 状态和潜在突变的筛选。随后对不同外显子的测序显示无突变。
我们在良性和恶性腮腺肿瘤中均未检测到 p53 的突变，因此我们假设 p53 在多形性腺瘤向癌在多形性腺瘤的转化中不起作用。
