Zhang Yong, Gao Yun, Xi Li-Sen, Deng Lei, Yin Ai-Hong, Wang Xue-Hao, Sun Bei-Cheng
Liver Transplantation Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Zhonghua Zhong Liu Za Zhi. 2009 Jun;31(6):410-3.
To study the efficacy of anti-telomerase siRNA in hepatocellular carcinoma both in vitro and in vivo.
Lentvirus vectors contained anti-telomerase siRNA were conducted with a high performance homologous recombination system, and then were transduced into human hepatocellular carcinoma HepG2 cells. The telomerase activity was detected by RT-PCR, HepG2 cell proliferation was determined by MTT assay, and apoptosis was detected by TUNEL assay. The in vivo experiment was carried out by inoculation of HepG2 cells into nude mice and the tumor growth was measured and analyzed.
The growth of transfected HepG2 cells was significantly inhibited and the inhibition rate was 57.5% at the 8th day after transfection. The telomerase activity was significantly suppressed in vitro. The growth of transfected human hepatocellular HepG2 tumor in the nude mice was also significantly inhibited.
The results of this study demonstrate that the growth of hepatocellular carcinoma cells is effectively inhibited by transfection of anti-telomerase siRNA both in vitro and in vivo.
研究抗端粒酶小干扰RNA（siRNA）在体外和体内对肝癌的疗效。
利用高效同源重组系统构建携带抗端粒酶siRNA的慢病毒载体，然后将其转导至人肝癌HepG2细胞。采用逆转录聚合酶链反应（RT-PCR）检测端粒酶活性，采用噻唑蓝（MTT）法检测HepG2细胞增殖情况，采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法（TUNEL）检测细胞凋亡情况。通过将HepG2细胞接种到裸鼠体内进行体内实验，并对肿瘤生长情况进行测量和分析。
转染后的HepG2细胞生长受到显著抑制，转染后第8天的抑制率为57.5%。体外端粒酶活性也受到显著抑制。转染后的人肝癌HepG2肿瘤在裸鼠体内的生长也受到显著抑制。
本研究结果表明，转染抗端粒酶siRNA在体外和体内均能有效抑制肝癌细胞的生长。
