Hu Xiao-Rong, Han Su-Xia, Wang Tao, Zhang Ming-Ke, Chen Lei, Wen Fu-Qiang
Department of Respiratory Medicine, Division of Pulmonary Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Sep;40(5):885-8.
To investigate the expression of interleukin (IL)-8 in lung tissues from patients with chronic obstructive pulmonary disease (COPD) and its association with stages of COPD.
The levels of mRNA and protein of IL-8 were measured with semi-quantitative polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) in patients with mild COPD (n=21), patients with advanced COPD (n=15), and controls (n=15). The correlations between IL-8 levels and stages of COPD, lung function (FEV1/ FVC%, FEV1% pred) and cigarette smoking were analyzed with Pearson correlation analysis.
The levels of IL-8 mRNA and protein in the lung tissues of COPD patients were significantly higher than those in the control group (P<0.05). The patients with advanced COPD had higher levels of IL-8 mRNA and protein than the patients with mild COPD (P<0.05). The COPD patients who smoked had higher levels of IL-8 mRNA and protein than those who did not smoke (P<0.05). But no significant differences in the levels of IL-8 mRNA and protein were found between smokers and and nonsmokers who did not have COPD (P>0.05). Increased expression of IL-8 in patients with COPD was positively correlated with stages of COPD (r=0.81, P<0.05); negatively correlated with lung function (FEV1/FVC%, FEV1% pred) (r=-0.62, -0.56, P<0.05), and positively correlated with volumes of cigarette smoking (r=0.53, P<0.05).
IL-8 is associated with stages of COPD, which may serve as an indication for clinical progress. Cigarette smoking increases IL-8 expression in the lung tissues of COPD patients.
探讨慢性阻塞性肺疾病（COPD）患者肺组织中白细胞介素（IL）-8的表达及其与COPD分期的关系。
采用半定量聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）检测轻度COPD患者（n = 21）、重度COPD患者（n = 15）和对照组（n = 15）肺组织中IL-8的mRNA和蛋白水平。采用Pearson相关分析分析IL-8水平与COPD分期、肺功能（FEV1/FVC%，FEV1%预计值）及吸烟的相关性。
COPD患者肺组织中IL-8的mRNA和蛋白水平显著高于对照组（P<0.05）。重度COPD患者的IL-8 mRNA和蛋白水平高于轻度COPD患者（P<0.05）。吸烟的COPD患者的IL-8 mRNA和蛋白水平高于不吸烟患者（P<0.05）。但在无COPD的吸烟者和非吸烟者之间，IL-8的mRNA和蛋白水平无显著差异（P>0.05）。COPD患者IL-8表达增加与COPD分期呈正相关（r = 0.81，P<0.05）；与肺功能（FEV1/FVC%，FEV1%预计值）呈负相关（r = -0.62，-0.56，P<0.05），与吸烟量呈正相关（r = 0.53，P<0.05）。
IL-8与COPD分期相关，可作为临床病情进展的指标。吸烟会增加COPD患者肺组织中IL-8的表达。
