Guo Jun-Tang, Ding Li-Hua, Liang Chao-Yang, Zhou Nai-Kang, Ye Qi-Nong
Department of Thoracic Surgery, PLA General Hospital, Beijing 100853, China.
Zhonghua Zhong Liu Za Zhi. 2009 Jul;31(7):528-31.
To identify the expression of Drosophila Eyes Absent Homologue 2 (EYA2) in non-small cell lung cancer (NSCLC) and to investigate its correlation with clinical parameters.
59 fresh specimens of lung cancer and paired normal lung tissue were obtained from 59 NSCLC cases treated in the department of thoracic surgery in our hospital from June 2006 to October 2007. Western blotting and immunohistochemistry were used to assay the specimens with goat anti-human EYA2 polyclone antibody. Clinicopathological parameters were collected and the correlation with EYA2 expression was subsequently analyzed.
The expression of EYA2 was detected in cytoplasm and nucleus of the cancer cells, but mostly in cytoplasm. Western blotting and immunohistochemistry showed the expression of EYA2 in NSCLC was increased and correlated with pathological type, but not with gender, age, pTNM stage, histological differentiation and lymph node metastasis. EYA2 expression was significantly up-regulated in adenocarcinoma, while not changed in lung squamous cell carcinoma.
The results of this study suggest that expression of EYA2 in lung adenocarcinoma is augmented. EYA2 is likely participating in the development of lung adenocarcinoma as a transcriptional activator.
检测果蝇无眼同源物2（EYA2）在非小细胞肺癌（NSCLC）中的表达情况，并探讨其与临床参数的相关性。
收集2006年6月至2007年10月在我院胸外科接受治疗的59例NSCLC患者的59份新鲜肺癌组织标本及配对的正常肺组织标本。采用蛋白质免疫印迹法（Western blotting）和免疫组织化学法，用羊抗人EYA2多克隆抗体检测标本。收集临床病理参数，随后分析其与EYA2表达的相关性。
在癌细胞的细胞质和细胞核中均检测到EYA2的表达，但主要在细胞质中。蛋白质免疫印迹法和免疫组织化学法显示，NSCLC中EYA2的表达增加，且与病理类型相关，但与性别、年龄、pTNM分期、组织学分化及淋巴结转移无关。腺癌中EYA2表达显著上调，而肺鳞癌中EYA2表达无变化。
本研究结果提示，EYA2在肺腺癌中的表达增强。EYA2可能作为一种转录激活因子参与肺腺癌的发生发展。
