Morton Alison J, Grosche Astrid, Rötting Anna K, Matyjaszek Sarah A, Blikslager Anthony T, Freeman David E
Island Whirl Equine Colic Research Laboratory, Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
Am J Vet Res. 2009 Dec;70(12):1536-44. doi: 10.2460/ajvr.70.12.1536.
OBJECTIVE-To identify expression and localization of cyclooxygenase (COX)-1 and COX-2 in healthy and ischemic-injured left dorsal colon of horses. SAMPLE POPULATION-Left dorsal colon tissue samples from 40 horses. PROCEDURES-Tissue samples that were used in several related studies on ischemia and reperfusion were evaluated. Samples were collected during anesthesia, before induction of ischemia, and following 1 hour of ischemia, 1 hour of ischemia and 30 minutes of reperfusion, 2 hours of ischemia, 2 hours of ischemia and 30 minutes of reperfusion, and 2 hours of ischemia and 18 hours of reperfusion. Histomorphometric analyses were performed to characterize morphological injury. Immunohistochemical analyses were performed to characterize expression and localization of COX-1 and COX-2. RESULTS-COX-1 and COX-2 were expressed in control tissues before ischemia was induced, predominantly in cells in the lamina propria. Ischemic injury significantly increased expression of COX-2 in epithelial cells on the colonic surface and in crypts. A similar significant increase of COX-1 expression was seen in the epithelial cells. CONCLUSIONS AND CLINICAL RELEVANCE-On the basis of information on the role of COX-2, upregulation of COX-2 in surface epithelium and crypt cells following ischemic injury in equine colon may represent an early step in the repair process.
目的——鉴定环氧化酶（COX）-1和COX-2在健康和缺血损伤的马左背侧结肠中的表达及定位。样本群体——40匹马的左背侧结肠组织样本。程序——对在多项关于缺血和再灌注的相关研究中使用的组织样本进行评估。样本在麻醉期间、缺血诱导前、缺血1小时后、缺血1小时和再灌注30分钟后、缺血2小时后、缺血2小时和再灌注30分钟后以及缺血2小时和再灌注18小时后采集。进行组织形态计量学分析以表征形态学损伤。进行免疫组织化学分析以表征COX-1和COX-2的表达及定位。结果——在缺血诱导前的对照组织中COX-1和COX-2有表达，主要在固有层细胞中。缺血损伤显著增加了结肠表面上皮细胞和隐窝中COX-2的表达。在上皮细胞中也观察到COX-1表达有类似的显著增加。结论及临床意义——基于关于COX-2作用的信息，马结肠缺血损伤后表面上皮和隐窝细胞中COX-2的上调可能代表修复过程的早期步骤。
