Odo Junichi, Sumihiro Manabu, Okadome Takuma, Inoguchi Masahiko, Akashi Haruo, Nakagoe Kazunori
Okayama University of Science, Ridai-cho, Kita-ku, Japan.
Chem Pharm Bull (Tokyo). 2009 Dec;57(12):1400-4. doi: 10.1248/cpb.57.1400.
A new water-insoluble Fe(3+)-TCAS[4]/TMPyP complex linked tetraanionic Fe(III)-thiacalix[4]arenetetrasulfonate (Fe(3+)-TCAS[4]) with tetracationic tetrakis(1-methylpyridinium-4-yl)porphine (TMPyP) via ionic interaction was prepared. The peroxidase-like catalytic activity of the Fe(3+)-TCAS[4]/TMPyP complex was investigated based on the dye formation reaction by oxidation of 4-aminoantipyrine and phenol with H(2)O(2) catalyzed by peroxidase. This Fe(3+)-TCAS[4]/TMPyP complex showed the highest activity in pH 5.5 acetate buffer solutions, and it was applied to the photometric determination of trace amounts of H(2)O(2). The calibration curve was linear over the range from 1.0 to 35 microg of H(2)O(2) in a 1.0 ml sample solution. Moreover, the method using glucoseoxidase and the Fe(3+)-TCAS[4]/TMPyP complex was applied to the determination of glucose, and the results were satisfactory even in control sera. The Fe(3+)-TCAS[4]/TMPyP complex can be applied to a practical sample, such as blood or urine, as an analytical reagent for the photometric determination of H(2)O(2) in place of peroxidase.
通过离子相互作用制备了一种新的水不溶性Fe(3+)-TCAS[4]/TMPyP复合物，它将四阴离子铁(III)-硫杂杯[4]芳烃四磺酸盐(Fe(3+)-TCAS[4])与四阳离子四(1-甲基吡啶-4-基)卟啉(TMPyP)连接起来。基于过氧化物酶催化H(2)O(2)氧化4-氨基安替比林和苯酚的染料形成反应，研究了Fe(3+)-TCAS[4]/TMPyP复合物的过氧化物酶样催化活性。该Fe(3+)-TCAS[4]/TMPyP复合物在pH 5.5的醋酸盐缓冲溶液中表现出最高活性，并将其应用于痕量H(2)O(2)的光度测定。在1.0 ml样品溶液中，校准曲线在1.0至35 μg H(2)O(2)范围内呈线性。此外，将使用葡萄糖氧化酶和Fe(3+)-TCAS[4]/TMPyP复合物的方法应用于葡萄糖的测定，即使在对照血清中结果也令人满意。Fe(3+)-TCAS[4]/TMPyP复合物可以作为一种分析试剂，代替过氧化物酶应用于实际样品，如血液或尿液中H(2)O(2)的光度测定。
