Franz T
Department of Neuroanatomy, Institute of Anatomy, University Hospital Eppendorf, Hamburg, Federal Republic of Germany.
Exp Hematol. 1991 Mar;19(3):170-3.
Osteopetrotic mutant mice (op/op) are deficient in osteoclasts and macrophages. In this report, the establishment of clonal permanent fibroblastoid cell lines from spleens of op/+ and op/op mice is described and conditioned medium obtained from these cell lines examined for release of growth factor that stimulates the proliferation and differentiation of macrophage precursor cells. It is shown that conditioned medium of op/+ fibroblastoid cells contains CSF-1 activity, whereas conditioned medium of op/op fibroblastoid cells does not. Conditioned medium of op/op cell lines does not inhibit colony-stimulating factor 1 (CSF-1) activity in conditioned medium of op/+ cells. Hybrids between op/op and op/+ fibroblastoid cells release CSF-1 activity in amounts similar to op/+ cell lines alone. These results confirm that op/op mice do not produce any CSF-1 activity. The data further suggest that predicted truncated proteins expressed from the mutated CSF-1 gene in op/op mice are functionally not relevant and that there is no evidence for trans-acting factors in op/op that might inhibit CSF-1 expression.
骨石化突变小鼠（op/op）缺乏破骨细胞和巨噬细胞。在本报告中，描述了从op/+和op/op小鼠脾脏建立克隆性永久性成纤维样细胞系，并检测了从这些细胞系获得的条件培养基中刺激巨噬细胞前体细胞增殖和分化的生长因子释放情况。结果表明，op/+成纤维样细胞的条件培养基含有集落刺激因子-1（CSF-1）活性，而op/op成纤维样细胞的条件培养基则没有。op/op细胞系的条件培养基不会抑制op/+细胞条件培养基中的集落刺激因子1（CSF-1）活性。op/op与op/+成纤维样细胞之间的杂交体释放的CSF-1活性量与单独的op/+细胞系相似。这些结果证实op/op小鼠不产生任何CSF-1活性。数据进一步表明，op/op小鼠中突变的CSF-1基因表达的预测截短蛋白在功能上不相关，并且没有证据表明op/op中有可能抑制CSF-1表达的反式作用因子。
