Yanabu M, Suzuki M, Soga T, Sone N, Nagata H, Nomura S, Kokawa T, Yasunaga K
First Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
Eur J Haematol. 1991 Feb;46(2):101-6. doi: 10.1111/j.1600-0609.1991.tb00529.x.
We investigated the characteristics of the antiplatelet autoantibodies in 60 patients with ITP. Using flow cytometry, the binding of monoclonal antibodies to the platelet glycoprotein (GP) IIb/IIIa complex and to GPIb was examined in these patients. The extent of binding was decreased in 15 patients (anti-GPIIb/IIIa in 12 patients and both anti-GPIIb/IIIa and anti-GPIb in 3 patients). Western blotting revealed that 10 of these 15 patients had either anti-GPIIb or anti-GPIIIa and 2 had anti-GPIb autoantibodies, ADP-induced aggregation of normal platelets was inhibited by autoantibodies in 12 of 60 patients, and 11 of these had anti-GPIIb/IIIa antibodies. Ristocetin-induced aggregation was inhibited in 4 of these patients, and 2 with prominent inhibition had anti-GPIb antibodies. There was a significant relationship between platelet-associated IgG value and ATP secretion. These results suggest that some antiplatelet autoantibodies can affect platelet function and thus have an influence on the pathophysiology of ITP.
我们研究了60例免疫性血小板减少症（ITP）患者抗血小板自身抗体的特征。使用流式细胞术，检测了这些患者中单克隆抗体与血小板糖蛋白（GP）IIb/IIIa复合物及GPIb的结合情况。15例患者（12例抗GPIIb/IIIa，3例抗GPIIb/IIIa和抗GPIb）的结合程度降低。蛋白质印迹法显示，这15例患者中有10例具有抗GPIIb或抗GPIIIa，2例具有抗GPIb自身抗体。60例患者中有12例患者的自身抗体抑制了正常血小板的ADP诱导聚集，其中11例具有抗GPIIb/IIIa抗体。这些患者中有4例患者的瑞斯托菌素诱导聚集受到抑制，其中2例抑制显著的患者具有抗GPIb抗体。血小板相关IgG值与ATP分泌之间存在显著关系。这些结果表明，某些抗血小板自身抗体可影响血小板功能，进而对ITP的病理生理学产生影响。
