Tan Sik-Loo, Sulaiman Sofiah, Pingguan-Murphy Belinda, Selvaratnam L, Tai Cheh-Chin, Kamarul T
Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Cell Tissue Bank. 2011 Feb;12(1):59-70. doi: 10.1007/s10561-009-9164-x. Epub 2009 Dec 2.
This study investigates the feasibility of processed human amnion (HAM) as a substrate for chondrogenic differentiation of mesenchymal stem cells (MSCs). HAM preparations processed by air drying (AD) and freeze drying (FD) underwent histological examination and MSC seeding in chondrogenic medium for 15 days. Monolayer cultures were used as control for chondrogenic differentiation and HAMs without cell seeding were used as negative control. Qualitative observations were made using scanning electron microscopy analysis and quantitative analyses were based on the sulfated glycosaminoglycans (GAG) assays performed on day 1 and day 15. Histological examination of HAM substrates before seeding revealed a smooth surface in AD substrates, while the FD substrates exhibited a porous surface. Cell attachment to AD and FD substrates on day 15 was qualitatively comparable. GAG were significantly highly expressed in cells seeded on FD HAM substrates. This study indicates that processed HAM is a potentially valuable material as a cell-carrier for MSC differentiation.
本研究调查了经处理的人羊膜（HAM）作为间充质干细胞（MSC）软骨分化底物的可行性。对通过空气干燥（AD）和冷冻干燥（FD）处理的HAM制剂进行组织学检查，并将MSC接种于软骨形成培养基中培养15天。单层培养用作软骨分化的对照，未接种细胞的HAM用作阴性对照。使用扫描电子显微镜分析进行定性观察，并基于第1天和第15天进行的硫酸化糖胺聚糖（GAG）测定进行定量分析。接种前对HAM底物的组织学检查显示，AD底物表面光滑，而FD底物表面呈现多孔状。第15天时，细胞在AD和FD底物上的附着在定性上具有可比性。在接种于FD HAM底物上的细胞中，GAG表达显著升高。本研究表明，经处理的HAM作为MSC分化的细胞载体是一种潜在的有价值材料。
