Frontier Research Base for Global Young Researchers, Frontier Research Center, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
J Biophotonics. 2010 Jun;3(5-6):368-72. doi: 10.1002/jbio.200900082.
Apoptosis-related microRNAs (miRNAs), the expression levels of which were observed to increase after photodynamic therapy (PDT), were identified as markers of PDT efficacy. miRNAs form a class of small endogenous RNAs that play important regulatory roles by targeting mRNA for cleavage or translational repression. miRNAs contribute to diverse biological processes, including development, cell growth, apoptosis, and hematopoiesis, which suggests a role in cancer development. However, no previous reports have described a relationship between apoptosis and miRNA expression after PDT. In this study, apoptosis-related miRNA levels were characterized by real-time PCR after PDT in HeLa cells. miR-210 and miR-296 expression levels increased 1 hour after PDT, although the other apoptosis-related miRNA (miR-7, miR-148a, miR-204, and miR-216) expression levels were unchanged. Since miR-210 is the most prominent miRNA consistently stimulated under hypoxic conditions and VEGF is capable of increasing miR-296 expression levels, these data suggest that hypoxia induced by PDT induces miR-210 expression, followed by an increase of VEGF expression and miR-296 expression. These results constitute the first report of an analysis of miRNA expression after PDT using talaporfin sodium as a photosensitizer, demonstrating that miR-210 and miR-296 expression levels are markers of PDT efficacy in HeLa cells.
光动力疗法 (PDT) 后观察到凋亡相关 microRNAs (miRNAs) 的表达水平增加，这些 miRNAs 被认为是 PDT 疗效的标志物。miRNAs 是一类小的内源性 RNA，通过靶向 mRNA 的切割或翻译抑制来发挥重要的调节作用。miRNAs 参与多种生物学过程，包括发育、细胞生长、凋亡和造血，这表明它们在癌症发展中发挥作用。然而，以前没有报道描述过 PDT 后凋亡与 miRNA 表达之间的关系。在这项研究中，通过实时 PCR 分析了 HeLa 细胞 PDT 后凋亡相关 miRNA 的水平。miR-210 和 miR-296 的表达水平在 PDT 后 1 小时增加，尽管其他凋亡相关 miRNA（miR-7、miR-148a、miR-204 和 miR-216）的表达水平没有变化。由于 miR-210 是在缺氧条件下最显著的 miRNA，并且 VEGF 能够增加 miR-296 的表达水平，这些数据表明 PDT 诱导的缺氧诱导 miR-210 的表达，随后 VEGF 表达和 miR-296 的表达增加。这些结果构成了使用他拉泊芬钠作为光敏剂分析 PDT 后 miRNA 表达的第一个报告，表明 miR-210 和 miR-296 的表达水平是 HeLa 细胞中 PDT 疗效的标志物。
