Faculty of Sciences, University of L'Aquila, gc-LNGS INFN, INFM Coppito, 67010 L'Aquila, Italy.
Microsc Res Tech. 2010 Jun;73(6):638-49. doi: 10.1002/jemt.20805.
This study is related to the application of the X-ray dual-energy microradiography technique together with the atomic absorption spectroscopy (AAS) for the detection of lead on Zea mays stem, ear, root, and leaf samples. To highlight the places with lead intake, the planar radiographs taken with monochromatic X-ray radiation in absorption regime with photon energy below and above the absorption edge of a given chemical element, respectively, are analyzed and processed. To recognize the biological structures involved in the intake, the dual-energy images with the lead signal have been compared with the optical images of the same Z. mays stem. The ear, stem, root, and leaf samples have also been analyzed with the AAS technique to measure the exact amount of the hyperaccumulated lead. The AAS measurement revealed that the highest intake occurred in the roots while the lowest in the maize ears and in the leaf. It seems there is a particular mechanism that protects the seeds and the leaves in the intake process.
本研究涉及 X 射线双能微辐射技术与原子吸收光谱法（AAS）的应用，用于检测玉米秸秆、穗、根和叶片样本中的铅。为了突出铅摄入的部位，分别用低于和高于特定化学元素吸收边的单能 X 射线辐射进行吸收模式的平面射线照相，并对所拍摄的射线照片进行分析和处理。为了识别参与摄入的生物结构，将具有铅信号的双能图像与同一 Z. mays 秸秆的光学图像进行了比较。还对玉米穗、秸秆、根和叶片样本进行了 AAS 技术分析，以测量超积累铅的确切含量。AAS 测量表明，根部的铅摄入量最高，而玉米穗和叶片的铅摄入量最低。在摄入过程中，似乎有一种特殊的机制可以保护种子和叶子。
