Microstructures and Microenvironments Research Group, Department of Chemical Engineering, School of Engineering and Physical Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland, UK.
Biotechnol Bioeng. 2010 Apr 1;105(5):945-54. doi: 10.1002/bit.22615.
It is shown that dielectrophoresis--the movement of particles in non-uniform electric fields--can be used to create engineered skin with artificial placodes of different sizes and shapes, in different spatial patterns. Modeling of the electric field distribution and image analysis of the cell aggregates produced showed that the aggregation is highly predictable. The cells in the aggregates remain viable, and reorganization and compaction of the cells in the aggregates occurs when the artificial skin is subsequently cultured. The system developed could be of considerable use for the in vitro study of developmental processes where local variations in cell density and direct cell-cell contacts are important.
研究表明，介电泳（即颗粒在非均匀电场中的运动）可用于创建具有不同大小和形状的人工基板的工程皮肤，且基板的空间模式也不同。对所产生的细胞聚集体的电场分布进行建模和图像分析表明，聚集过程具有高度可预测性。聚集体中的细胞保持存活，并且当随后对人工皮肤进行培养时，聚集体中的细胞会发生重组和浓缩。开发的系统对于体外研究发育过程可能非常有用，因为在这些过程中，细胞密度的局部变化和直接的细胞-细胞接触很重要。
