Paz G, Yogev L, Gottreich A, Rotem R, Yavetz H, Homonnai Z T
Serlin Maternity Hospital, Tel Aviv Medical Center, Sackler Medical School, Tel Aviv University, Israel.
Eur J Obstet Gynecol Reprod Biol. 1991 Jan 30;38(2):141-4. doi: 10.1016/0028-2243(91)90191-m.
The use of an electric freezer for cryostorage of human semen is described. A simple method for semen freezing in liquid nitrogen after dilution with 7.5% glycerol was used. Thawing for quality analysis revealed only a small decrease (10%) in post-thaw sperm motility (59 +/- 1.4 vs. 53 +/- 1.8%; mean +/- SE, n = 12, P less than 10(-5)) and 14% in post-thaw sperm vitality (85.0 +/- 1.3 vs. 72.9 +/- 1.8%). After the freezing process, the samples, three of each donor, were cryopreserved in a regular electric freezer which maintained temperatures in the range of -85 degrees C (+/- 2 degrees C). The samples were stored for 1 week, 2 and 6 months, thawed and then assayed for motility and vitality. No effect of storage was found for a period up to 2 months. An additional decrease of 17.2% in sperm motility and 18% in sperm vitality were noted only after 6 months of preservation. The final motility and vitality rates of these sperm samples were 44 +/- 2.4 and 60 +/- 3.0%, respectively. According to these results, in cases of sperm storage for limited periods, it is recommended to cryopreserve human semen by the use of a combination of freezing in liquid nitrogen and storage of the samples in an electric freezer at -85 degrees C.
本文描述了使用电动冷冻箱进行人类精液冷冻保存的方法。采用了一种简单的方法，即在精液用7.5%甘油稀释后在液氮中冷冻。解冻后进行质量分析发现，解冻后精子活力仅略有下降（10%）（解冻后精子活力为59±1.4%，而解冻前为53±1.8%；均值±标准误，n = 12，P<10⁻⁵），解冻后精子存活率下降了14%（解冻后为85.0±1.3%，解冻前为72.9±1.8%）。冷冻过程完成后，每个供体的三份样本被保存在一个普通的电动冷冻箱中，该冷冻箱能将温度维持在-85℃（±2℃）范围内。样本分别保存1周、2个月和6个月，然后解冻并检测活力和存活率。在长达2个月的保存期内未发现保存的影响。仅在保存6个月后，精子活力又额外下降了17.2%，精子存活率下降了18%。这些精子样本最终的活力率和存活率分别为44±2.4%和60±3.0%。根据这些结果，在精子需要短期保存的情况下，建议采用液氮冷冻与在-85℃的电动冷冻箱中保存样本相结合的方法来冷冻保存人类精液。
