Wang Tian, Zhao Yi, Liu Xu, Liu Xia, Li Ru, Ren Li-min, Ye Hua, Li Zhan-guo
Department of Rheumatology and Immunology, the People's Hospital, Peking University Medical School, Beijing 100044, China.
Zhonghua Yi Xue Za Zhi. 2009 Jul 21;89(27):1885-8.
To identify interleukin-17 (IL-17 )-producing CD8 positive T cells from patients with rheumatoid arthritis (RA), and investigate its cytokines as well as their correlations.
Peripheral blood got from 39 RA patients and 40 healthy donors. Flow cytometry was used to analyze the subsets of T cells in peripheral blood from these 39 RA patients and 27 healthy donors. 11-17, IL-6, and IL-23 levels in sera of all these people were tested by ELISA.
The median of CD8 IL-17+ T cells from RA patients was 2.7% (95% confidence internal was 1.55%-3.74%); the median of these cells from healthy controls was 1.61% (95% confidence internal was 1.25%-2.61%). CD8+ IL-17+ T cells elevated significantly in RA(P < 0.05). As the production of CD8+ IL-17+ T cells, the IL-17 levels in sera of RA patients and healthy controls were (429 +/- 502) ng/L and (13 +/- 30) ng/L respectively. The IL-17 level in RA is higher than that in healthy people (P < 0.01). Meanwhile, to constitute CD8+ IL-17+ T cell polarizing condition, IL-23 and IL-6 in sera of RA were (157.83 +/- 27.07) ng/L and (32.67 +/- 34.50) x ng/L individually. These two cytokines in healthy controls were (21.97 +/- 3.52) ng/L and (1.82 +/- 1.51) ng/L severally. IL-6 and IL-23 increased clearly in peripheral blood of RA patients as compared with healthy donors (P < 0.01).
This study suggested that elevated CD8+ IL-17+ T cells which involved in IL-17 secretion may depend on increased IL-6 and IL-23 in peripheral blood of rheumatoid arthritis.
鉴定类风湿关节炎（RA）患者中产生白细胞介素 - 17（IL - 17）的CD8阳性T细胞，并研究其细胞因子及其相关性。
采集39例RA患者和40例健康供者的外周血。采用流式细胞术分析这39例RA患者和27例健康供者外周血中T细胞亚群。采用酶联免疫吸附测定法检测所有这些人的血清中IL - 17、IL - 6和IL - 23水平。
RA患者CD8 IL - 17 + T细胞的中位数为2.7%（95%置信区间为1.55% - 3.74%）；健康对照者这些细胞的中位数为1.61%（95%置信区间为1.25% - 2.61%）。RA患者中CD8 + IL - 17 + T细胞显著升高（P < 0.05）。作为CD8 + IL - 17 + T细胞的产物，RA患者和健康对照者血清中的IL - 17水平分别为（429 ± 502）ng/L和（13 ± 30）ng/L。RA患者的IL - 17水平高于健康人（P < 0.01）。同时，为构成CD8 + IL - 17 + T细胞极化条件，RA患者血清中的IL - 23和IL - 6分别为（157.83 ± 27.07）ng/L和（32.67 ± 34.50）ng/L。健康对照者血清中这两种细胞因子分别为（21.97 ± 3.52）ng/L和（1.82 ± 1.51）ng/L。与健康供者相比，RA患者外周血中IL - 6和IL - 23明显升高（P < 0.01）。
本研究提示，参与IL - 17分泌的CD8 + IL - 17 + T细胞升高可能与类风湿关节炎患者外周血中IL - 6和IL - 23增加有关。
