[高脂血症兔离体阴茎海绵体收缩性与舒张性的变化]
[Changes of contractility and relaxation of corpus cavernosum in hyperlipidemic rabbit in vitro].
作者信息
Rao Ke, Liu Ji-Hong, Chen Rui-Bao, Zhang Hui-Ping, Wang Tao, Wang Shao-Gang, Yang Wei-Min, Ye Zhang-Qun
机构信息
Department of Urology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2009 Jul 14;89(26):1854-7.
OBJECTIVE
To investigate the mechanisms of erectile dysfunction induced by hyperlipidemia through studying the changes of contractility and relaxation of corpus cavernosum in hypercholesterolemic rabbit in vitro.
METHODS
New Zealand white rabbits were randomly divided into control and experiment groups. The control group (n = 20) received a regular diet for 8 weeks while the experimental group (n = 20) were fed a 1% cholesterol diet for 8 weeks. The authors conducted isometric tension studies with phenylephrine, endothelium-dependent vasodilators (acetylcholine), endothelium-independent vasodilators (sodium nitroprusside) and rho kinase inhibitor (Fasudil) on isolated strips of corpus cavernosum.
RESULTS
The weight and total serum cholesterol significantly improved (P < 0.01) in experimental group as compared with the non-fed experimental group. The total serum cholesterol significantly improved (P < 0.01) in experimental group as compared with the control group. The contractility responses to phenylephrine in experiment group in doses (0.5, 1, 5, 10, 50, 100 micromol) were 4.79% +/- 2.00%, 8.84% +/-2.95%, 12.81% +/- 3.77%, 14.63% +/- 5.38%, 25.01% +/- 6.14% and 34.69% +/- 8.53% respectively. The contractility responses to phenylephrine in control group were 1.00% +/- 0.3%, 2.60% +/- 0.72%, 4.28% +/- 1.27%, 5.91% +/- 2.09%, 6.49% +/- 4.02% and 5.64% +/- 11.87% respectively. The contractility responses to phenylephrine significantly improved (P < 0.01) in experimental group in these doses as compared with the control group. The relaxation responses to acetylcholine in experimental group in doses (1, 10 and 100 micromol) were 36.28% +/- 4.71%, 48.81% +/- 4.36% and 56.27% +/- 11.93% respectively. The relaxation responses in control group were 48.04% +/- 4.78%, 69.12% +/- 5.27% and 78.23% +/- 5.30% respectively. There was significant reduction (P < 0.01) in experimental group in these doses as compared with the control group. No difference were found among the two groups in the relaxation response to sodium nitroprusside. The relaxation responses to fasudil in experimental group in doses (0.25, 1.25, 2.5 and 12.5 micromol) were 1.56% +/- 0.43%, 5.03% +/- 1.02%, 8.28% +/- 1.35% and 16.77% +/- 3.57% respectively. The relaxation responses in control group were 4.69% +/- 1.23%, 10.39% +/- 2.05%, 15.08% +/- 3.04% and 25.22% +/- 3.72% respectively. There was significant reduction (P < 0.01) in experimental group in these doses as compared with the control group.
CONCLUSION
The improvement of cavernous smooth muscle contractility and the impairment of cavernous smooth muscle relaxation in response to endothelium-mediated stimuli are the mechanisms of erectile dysfunction induced by hyperlipidemia.
目的
通过研究高胆固醇血症兔离体海绵体收缩和舒张功能的变化，探讨高脂血症诱发勃起功能障碍的机制。
方法
将新西兰白兔随机分为对照组和实验组。对照组（n = 20）给予常规饮食8周，而实验组（n = 20）给予1%胆固醇饮食8周。作者对离体海绵体条带进行等长张力研究，使用去氧肾上腺素、内皮依赖性血管舒张剂（乙酰胆碱）、非内皮依赖性血管舒张剂（硝普钠）和 Rho 激酶抑制剂（法舒地尔）。
结果
与未喂食实验组相比，实验组体重和总血清胆固醇显著改善（P < 0.01）。与对照组相比，实验组总血清胆固醇显著改善（P < 0.01）。实验组对去氧肾上腺素剂量（0.5、1、5、10、50、100 μmol）的收缩反应分别为4.79%±2.00%、8.84%±2.95%、12.81%±3.77%、14.63%±5.38%、25.01%±6.14%和34.69%±8.53%。对照组对去氧肾上腺素的收缩反应分别为1.00%±0.3%、2.60%±0.72%、4.28%±1.27%、5.91%±2.09%、6.49%±4.02%和5.64%±11.87%。与对照组相比，实验组在这些剂量下去氧肾上腺素的收缩反应显著改善（P < 0.01）。实验组对乙酰胆碱剂量（1、10和100 μmol）的舒张反应分别为36.28%±4.71%、48.81%±4.36%和56.27%±11.93%。对照组的舒张反应分别为48.04%±4.78%、69.12%±5.27%和78.23%±5.30%。与对照组相比，实验组在这些剂量下有显著降低（P < 0.01）。两组对硝普钠的舒张反应无差异。实验组对法舒地尔剂量（0.25、1.25、2.5和12.5 μmol）的舒张反应分别为1.56%±0.43%、5.03%±1.02%、8.28%±1.35%和16.77%±3.57%。对照组的舒张反应分别为4.69%±1.23%、10.39%±2.05%、15.08%±3.04%和25.22%±3.72%。与对照组相比，实验组在这些剂量下有显著降低（P < 0.01）。
结论
海绵体平滑肌收缩性增强以及对内皮介导刺激的海绵体平滑肌舒张功能受损是高脂血症诱发勃起功能障碍的机制。
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