Li Qing-Shu, Qi Hong-Bo
Department of Obstetrics and Gynecology, First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Zhonghua Fu Chan Ke Za Zhi. 2009 Jun;44(6):405-8.
To study the relationship between bone morphogenetic protein-2 (BMP-2) and premature rupture of membranes (PROM).
Ninety gravidas giving birth at 4 hospitals in Chongqing from January 2006 to January 2007 were selected and divided into 5 groups (18 in each): term labor group ( with intact membranes), term PROM group, preterm labor group (with intact membranes), preterm PROM (PPROM) group and control group (term pregnancy not in labor). The expression of BMP-2 in the chorioamniotic membranes of each group were detected by streptavidin-peroxidase methods and its relationship with PROM plus histological chorioamnionitis were analyzed.
(1) BMP-2 was detected in the cytoplasm of epithelial and interstitial cells of membranes in each group. (2) BMP-2 expression in term labor group, term PROM group, preterm labor group and PPROM group were 0.0137 +/- 0.0094, 0.0269 +/- 0.0127, 0.0273 +/- 0.0145, 0.0456 +/- 0.0114, respectively, which higher than that of the control group 0.0060 +/- 0.0079 (P < 0.01). BMP-2 expression in the term PROM group was higher than that of the term labor group (P < 0.01), that in the PPROM was higher than in the preterm labor group and term PROM group (P < 0.01). (3) Pathological examination found that 17 out of the 90 women with histologic chorioamnionitis, of which 13 (36%) came from the term PROM and PPROM group with a higher level of BMP-2 expression than those without chorioamnionitis (0.0201 +/- 0.0107 vs 0.0105 +/- 0.0092, P < 0.05).
The expression of BMP-2 in chorioamniotic membranes is closely related to PROM.
研究骨形态发生蛋白-2（BMP-2）与胎膜早破（PROM）之间的关系。
选取2006年1月至2007年1月在重庆4家医院分娩的90例孕妇，分为5组（每组18例）：足月产组（胎膜完整）、足月胎膜早破组、早产组（胎膜完整）、早产胎膜早破（PPROM）组和对照组（足月妊娠未临产）。采用链霉亲和素-过氧化物酶法检测各组羊膜绒毛膜中BMP-2的表达，并分析其与胎膜早破合并组织学绒毛膜羊膜炎的关系。
（1）各组胎膜上皮细胞和间质细胞的胞质中均检测到BMP-2。（2）足月产组、足月胎膜早破组、早产组和PPROM组BMP-2表达分别为0.0137±0.0094、0.0269±0.0127、0.0273±0.0145、0.0456±0.0114，均高于对照组0.0060±0.0079（P<0.01）。足月胎膜早破组BMP-2表达高于足月产组（P<0.01），PPROM组高于早产组和足月胎膜早破组（P<0.01）。（3）病理检查发现90例孕妇中有17例组织学绒毛膜羊膜炎，其中13例（36%）来自足月胎膜早破组和PPROM组，其BMP-2表达水平高于无绒毛膜羊膜炎者（0.0201±0.0107 vs 0.0105±0.0092，P<0.05）。
羊膜绒毛膜中BMP-2表达与胎膜早破密切相关。
