[高脂饮食和追赶生长对大鼠胃排空及血浆胰高血糖素样肽-1浓度的影响]

[The effect of high-fat diet and catch-up growth on gastric emptying and plasma glucagon-like peptide-1 concentration in rats].

作者信息

Chen Lu-Lu, Yang Wei-Hong, Zheng Juan, Yue Ling, Hu Xiang

机构信息

Department of Endocrinology, Xiehe Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China.

出版信息

Zhonghua Nei Ke Za Zhi. 2009 Aug;48(8):655-9.

PMID:19954058

Abstract

OBJECTIVE

To investigate the effect of high-fat diet and catch-up growth on gastric emptying and plasma glucagon-like peptide-1 (GLP-1) concentration in rats.

METHODS

Sixty SD rats were randomly divided into four groups, i. e. normal control group (NC group), refed with normal diet group (RN group), high fat diet group (HF group) and refed with high fat diet group (RH group). The weight and diet of rats were recorded. At the onset of refeeding (4 weeks ) and after 2 or 4 weeks of refeeding (6 or 8 weeks), animals were sacrificed and then gastric emptying study test and the assessment of plasma GLP-1 concentration were carried out.

RESULTS

Compared with NC group, rats in HF group possessed accelerated gastric emptying rate (95.61% vs 96.16%, 94.24% vs 96.11%, 93.08% vs 95.50%) and increased GLP-1 concentration (12.02 pmol/L vs 16.27 pmol/L, 10.77 pmol/L vs 13.93 pmol/L, 9.47 pmol/L vs 11.53 pmol/L) at 4, 6, 8 weeks respectively. Similarly, compared with NC group, RN group possessed slowed gastric emptying rate (95.61% vs 87.60%) and decreased GLP-1 concentration (12.02 pmol/L vs 9.69 pmol/L) at 4 weeks, but accelerated gastric emptying rate (94.24% vs 96.11%, 93.08% vs 94.52%) and increased GLP-1 concentration (10.77 pmol/L vs 12.57 pmol/L, 9.47 pmol/L vs 9.80 pmol/L) at 6 and 8 weeks respectively. Compared with HF group, RH group obtained slowed gastric emptying rate (96.16% vs 90.70%) and decreased GLP-1 concentration (16.27 pmol/L vs 9.69 pmol/L) at 4 weeks, but accelerated gastric emptying rate(96.11% vs 98.02%) and slightly lower GLP-1 concentration (13.93 pmol/L vs 13.44 pmol/L) at 6 weeks, and further slowed gastric emptying rate (96.50% vs 92.57%) and lower GLP-1 concentration (11.53 pmol/L vs 10.00 pmol/L).

CONCLUSION

Although both high-fat diet and catch-up growth affect the gastrointestinal function, the latter has a greater impact, which could account for some pathophysiological changes caused by catch-up growth.

摘要

目的

探讨高脂饮食和追赶生长对大鼠胃排空及血浆胰高血糖素样肽-1（GLP-1）浓度的影响。

方法

将60只SD大鼠随机分为四组，即正常对照组（NC组）、恢复正常饮食组（RN组）、高脂饮食组（HF组）和高脂饮食后恢复组（RH组）。记录大鼠体重和饮食情况。在恢复喂食开始时（4周）以及恢复喂食2周或4周后（6周或8周），处死动物，然后进行胃排空研究试验并评估血浆GLP-1浓度。

结果

与NC组相比，HF组大鼠在4周、6周、8周时胃排空率加快（分别为95.61%对96.16%、94.24%对96.11%、93.08%对95.50%），GLP-1浓度升高（分别为12.02 pmol/L对16.27 pmol/L、10.77 pmol/L对13.93 pmol/L、9.47 pmol/L对11.53 pmol/L）。同样，与NC组相比，RN组在4周时胃排空率减慢（95.61%对87.60%），GLP-1浓度降低（12.02 pmol/L对9.69 pmol/L），但在6周和8周时胃排空率加快（分别为94.24%对96.11%、93.08%对94.52%），GLP-1浓度升高（分别为10.77 pmol/L对12.57 pmol/L、9.47 pmol/L对9.80 pmol/L）。与HF组相比，RH组在4周时胃排空率减慢（96.16%对90.70%），GLP-1浓度降低（16.27 pmol/L对9.69 pmol/L），但在6周时胃排空率加快（96.11%对98.02%），GLP-1浓度略低（13.93 pmol/L对13.44 pmol/L），在8周时胃排空率进一步减慢（96.50%对92.57%），GLP-1浓度更低（11.53 pmol/L对10.00 pmol/L）。