Institut für Strukturbiologie und Biophysik, Forschungszentrum Jülich, Jülich, Germany.
Rejuvenation Res. 2010 Apr-Jun;13(2-3):210-3. doi: 10.1089/rej.2009.0926.
Previously, two D-enantiomeric amino acid peptides, D1 and D3, which specifically bind to the amyloid-beta peptide Abeta(1-42), were identified by phage display selection. To assess the diagnostic and therapeutic potentials of D1 and D3 for the diagnosis and treatment of Alzheimer disease, the blood-brain barrier transport of these D-peptides was quantitatively evaluated in vitro. Our results showed that the apical-to-basolateral transport of D3 was more efficient than that of D1. An active efflux transport mechanism seems to oppose the transport of D1, whereas D3 is likely to be transported through the blood-brain barrier via an adsorptive-mediated transcytosis mechanism.
先前，通过噬菌体展示选择，鉴定出两种特异性结合淀粉样β肽 Abeta(1-42)的 D-对映体氨基酸肽 D1 和 D3。为了评估 D1 和 D3 用于诊断和治疗阿尔茨海默病的诊断和治疗潜力，在体外定量评估了这些 D-肽的血脑屏障转运。我们的结果表明，D3 的顶侧到基底外侧转运比 D1 更有效。主动外排转运机制似乎与 D1 的转运相反，而 D3 可能通过吸附介导的胞吞转运机制穿过血脑屏障进行转运。
