Dantas-Torres F, Lia R P, Barbuto M, Casiraghi M, Crovace A, Caligiani L, Genchi C, Otranto D
Prof D. Otranto Dipartimento di Sanitá Pubblica e Zootecnia, Universitá degli Studi di Bari, 70010 Bari, Italy.
J Small Anim Pract. 2009 Dec;50(12):667-9. doi: 10.1111/j.1748-5827.2009.00846.x.
A five-year-old, entire female mixed-breed dog was presented with corneal oedema and episcleral hyperaemia in the left eye. The ophthalmological examination revealed the presence of a free-swimming nematode in the anterior chamber. Circulating microfilariae were not observed by a modified Knott test nor were adult antigens detected in serum by a commercial ELISA. The parasite was surgically removed from the dog's eye, but its anterior end was damaged during the surgery. Based on the morphology of the posterior end, the nematode was preliminarily identified as a male Dirofilaria immitis. The species identification was confirmed by PCR amplification and sequencing of the mitochondrial coxI and 12S rDNA genes, using a DNA barcoding approach. Although other cases of ocular dirofilariosis by D. immitis have been previously recorded in Australia and the United States, the case reported herein is the first in a dog from Europe.
一只5岁的未绝育雌性混种犬因左眼角膜水肿和巩膜充血前来就诊。眼科检查发现前房内有一条自由游动的线虫。改良Knott试验未检测到循环微丝蚴，商业ELISA也未在血清中检测到成虫抗原。通过手术从犬眼中取出寄生虫，但手术过程中其前端受损。根据后端形态，该线虫初步鉴定为雄性犬恶丝虫。采用DNA条形码方法，通过线粒体细胞色素c氧化酶亚基I（coxI）和12S核糖体DNA（rDNA）基因的PCR扩增和测序，确认了物种鉴定。尽管此前在澳大利亚和美国曾有过犬恶丝虫引起眼部丝虫病的其他病例记录，但本文报道的病例是欧洲首例犬感染该病例。
