Liu Qiong, Xu Wei, Qiu Hai-rong, Wang Rong, Yu Hui, Fan Lei, Miao Kou-rong, Li Jian-yong
Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Zhonghua Xue Ye Xue Za Zhi. 2009 Sep;30(9):601-4.
To explore the effect of CpG-oligodeoxynucleotides (ODN) in chromosome study of chronic lymphocytic leukemia (CLL).
Blood or bone marrow cells of 70 CLL patients were cultured for 72 h with PHA, CpG-ODN and CpG-ODN combined with IL-2, respectively. Routine karyotype analysis with R banding technique and interphase fluorescence in situ hybridization (FISH) were performed.
The metaphase number>or=20 was considered as successful stimulation, which in PHA, CpG-ODN and CpG-ODN combined IL-2 groups were 90.0%, 68.6% and 68.6%, respectively, and the detection rates of chromosome aberrations were 3.2%, 43.6% and 43.6%, respectively. The aberrations rates detected by interphase FISH with a panel of probes was 64.3%.
CpG-ODN DSP30 can effectively raise the detection rate of chromosome aberrations in CLL patients.
