猪戊型肝炎病毒实验性感染猪肝脏中α干扰素和Mx蛋白的表达
Expression of interferon-alpha and Mx protein in the livers of pigs experimentally infected with swine hepatitis E virus.
作者信息
Lee Y H, Ha Y, Chae C
机构信息
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of South Korea.
出版信息
J Comp Pathol. 2010 Feb-Apr;142(2-3):187-92. doi: 10.1016/j.jcpa.2009.10.025. Epub 2009 Dec 1.
Expression of interferon-alpha (IFN-alpha) and Mx protein in the livers of pigs experimentally infected with swine hepatitis E virus (HEV) was examined immunohistochemically. Five infected and five non-infected pigs were killed at 3, 7, 14, 21, 29 and 50 days post-inoculation (dpi). Tissues were collected from each pig at necropsy. Multifocal lymphoplasmacytic hepatitis (P=0.042) and focal hepatocellular necrosis (P=0.037) were significantly more frequent in the swine HEV-infected pigs than in the non-infected control pigs. Immunohistochemical analysis detected expression of IFN-alpha and Mx protein by macrophages/Kupffer cells, lymphocytes and hepatocytes in the livers of infected pigs.
采用免疫组织化学方法检测了实验感染猪戊型肝炎病毒（HEV）的猪肝脏中α干扰素（IFN-α）和Mx蛋白的表达。在接种后3、7、14、21、29和50天处死5头感染猪和5头未感染猪。尸检时从每头猪采集组织。与未感染的对照猪相比，猪HEV感染猪的多灶性淋巴浆细胞性肝炎（P = 0.042）和局灶性肝细胞坏死（P = 0.037）明显更常见。免疫组织化学分析检测到感染猪肝脏中的巨噬细胞/枯否细胞、淋巴细胞和肝细胞表达IFN-α和Mx蛋白。
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