Institut National de la Santé et de la Recherche Médicale, INSERM, Unité 977, Faculté de Médecine, 11 Rue Humann, 67085 Strasbourg Cedex, France.
Biomaterials. 2010 Mar;31(7):1699-706. doi: 10.1016/j.biomaterials.2009.11.032. Epub 2009 Dec 1.
In this work, we designed replica particles based on poly (L-lysine) (PLL) polymers crosslinked via a homobifunctional linker to support coadsorption of a plasmid DNA and a peptide hormone for concurrent transfection and induction of a cellular function. PLL replica particles (PLL(RP)) were prepared by infiltrating polymer into mesoporous silica (MS) particles, crosslinking the adsorbed chains by using a homobifunctional crosslinker and finally removing the template particles. Moreover, we verified their cytotoxicity. Furthermore, based on this PLL(RP) gene delivery system, we simultaneously evaluated the melanin stimulation and gene expression in these cells by fluorescence microscopy. To further understand the bi-functionality, we labeled the SPT7pTL and PGA-alpha-MSH with YOYO-1 and Rhodamine, respectively, to follow its intracellular pathway by confocal microscopy. Our data suggests that the PLL(RP) is a promising vector for gene therapy and hormone stimulation.
在这项工作中，我们设计了基于聚（L-赖氨酸）（PLL）聚合物的复制颗粒，这些聚合物通过同双功能连接子交联，以支持质粒 DNA 和肽激素的共吸附，从而实现细胞功能的同时转染和诱导。PLL 复制颗粒（PLL（RP））通过将聚合物渗透到介孔硅（MS）颗粒中，然后使用同双功能交联剂交联吸附的链，最后去除模板颗粒来制备。此外，我们还验证了它们的细胞毒性。此外，基于这种 PLL（RP）基因传递系统，我们通过荧光显微镜同时评估了这些细胞中的黑色素刺激和基因表达。为了进一步了解双功能性，我们分别用 YOYO-1 和 Rhodamine 标记 SPT7pTL 和 PGA-alpha-MSH，通过共聚焦显微镜跟踪其细胞内途径。我们的数据表明，PLL（RP）是基因治疗和激素刺激的有前途的载体。
