同时 HPLC 分析液体制剂中的盐酸伪麻黄碱、磷酸可待因和盐酸曲普利啶。
Simultaneous HPLC analysis of pseudophedrine hydrochloride, codeine phosphate, and triprolidine hydrochloride in liquid dosage forms.
机构信息
Faculty of Science and Technology, Al-Quds University, P.O. Box 20002, East Jerusalem, Palestine.
出版信息
J Pharm Biomed Anal. 2010 Mar 11;51(4):991-3. doi: 10.1016/j.jpba.2009.10.024. Epub 2009 Nov 10.
An HPLC method using UV detection is proposed for the simultaneous determination of pseudophedrine hydrochloride, codeine phosphate, and triprolidine hydrochloride in liquid formulation. C18 column (250mmx4.0mm) is used as the stationary phase with a mixture of methanol:acetate buffer:acetonitrile (85:5:10, v/v) as the mobile phase. The factors affecting column separation of the analytes were studied. The calibration graphs exhibited a linear concentration range of 0.06-1.0mg/ml for pseudophedrine hydrochloride, 0.02-1.0mg/ml for codeine phosphate, and 0.0025-1.0mg/ml for triprolidine hydrochloride for a sample size of 5microl with correlation coefficients of better than 0.999 for all active ingredients studied. The results demonstrate that this method is reliable, reproducible and suitable for routine use with analysis time of less than 4min.
提出了一种 HPLC 方法，采用紫外检测法，可同时测定液体制剂中的盐酸伪麻黄碱、磷酸可待因和盐酸曲普利啶。使用 C18 柱（250mmx4.0mm）作为固定相，甲醇：乙酸缓冲液：乙腈（85:5:10，v/v）作为流动相。研究了影响分析物柱分离的因素。校准曲线显示，盐酸伪麻黄碱的线性浓度范围为 0.06-1.0mg/ml，磷酸可待因的线性浓度范围为 0.02-1.0mg/ml，盐酸曲普利啶的线性浓度范围为 0.0025-1.0mg/ml，进样量为 5μl，所有研究成分的相关系数均大于 0.999。结果表明，该方法可靠、重现性好，适用于常规使用，分析时间小于 4min。
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