Department of Gynecology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China.
Hum Reprod. 2010 Feb;25(2):392-7. doi: 10.1093/humrep/dep427. Epub 2009 Dec 1.
Although nerve fibres are present in eutopic and ectopic endometrium, it is unclear whether they appear in ovarian endometriotic lesions. We investigated the presence of nerve fibres in ovarian endometriotic lesions and its correlation with clinical parameters in women with ovarian endometriosis.
Histological sections of ovarian endometriotic lesions from 61 women with ovarian endometriosis (Stages II-IV) who underwent laparoscopic endometrioma cystectomy were stained immunohistochemically using a specific polyclonal rabbit anti-protein gene product 9.5 (PGP9.5) antibody to demonstrate myelinated and unmyelinated nerve fibres.
Nerve fibres stained with PGP9.5 were detected in ovarian endometriotic lesions in 31.1% of women, and most appeared in fibrotic interstitium of ovarian endometriotic lesions. The density of PGP9.5-immunoactive fibres in ovarian endometriotic lesions in women with pain symptoms (n = 35) was higher than in women with no pain symptoms (n = 26, P = 0.039), although the percentage (positive cases/total) of PGP9.5-positive fibres did not differ. In women with pain symptoms, PGP9.5-positive fibres appeared in 40.0% of cases and the density of PGP9.5-immunoactive fibres in ovarian endometriotic lesions was correlated with severity of pain symptoms (r = 0.466, P = 0.005). In women with no pain, PGP9.5-positive fibres were detected in only 5 (19.2%) women. Both the percentage and the density of PGP9.5-positive fibres in ovarian endometriotic lesions were associated with pelvic adhesions (chi2 = 6.833, P = 0.009; Z = 2.442, P = 0.015, respectively) but not with disease severity.
PGP9.5-immunoactive nerve fibres in ovarian endometriotic lesions may be involved in the pathophysiology of pain generation and pelvic adhesion formation in women with ovarian endometriosis.
尽管神经纤维存在于正常和异位子宫内膜中，但尚不清楚它们是否出现在卵巢子宫内膜异位症病变中。我们研究了神经纤维在卵巢子宫内膜异位症病变中的存在及其与卵巢子宫内膜异位症患者临床参数的相关性。
对 61 例接受腹腔镜卵巢子宫内膜囊肿切除术的卵巢子宫内膜异位症（Ⅱ-Ⅳ期）患者的卵巢子宫内膜异位症病变的组织学切片进行免疫组织化学染色，使用特定的多克隆兔抗蛋白基因产物 9.5（PGP9.5）抗体显示有髓和无髓神经纤维。
在 31.1%的卵巢子宫内膜异位症患者的卵巢子宫内膜异位症病变中检测到 PGP9.5 染色的神经纤维，并且大多数出现在卵巢子宫内膜异位症病变的纤维化间质中。有疼痛症状的妇女（n=35）卵巢子宫内膜异位症病变中 PGP9.5 免疫活性纤维的密度高于无疼痛症状的妇女（n=26，P=0.039），尽管 PGP9.5 阳性纤维的百分比（阳性病例/总数）没有差异。在有疼痛症状的妇女中，40.0%的病例出现 PGP9.5 阳性纤维，卵巢子宫内膜异位症病变中 PGP9.5 免疫活性纤维的密度与疼痛症状的严重程度相关（r=0.466，P=0.005）。在没有疼痛的妇女中，仅在 5 名妇女（19.2%）中检测到 PGP9.5 阳性纤维。卵巢子宫内膜异位症病变中 PGP9.5 阳性纤维的百分比和密度均与盆腔粘连有关（卡方=6.833，P=0.009；Z=2.442，P=0.015），但与疾病严重程度无关。
卵巢子宫内膜异位症病变中 PGP9.5 免疫活性神经纤维可能参与卵巢子宫内膜异位症患者疼痛发生和盆腔粘连形成的病理生理学过程。
