一种基于选择性结扎妊娠兔子宫胎盘血管的胎儿生长受限实验模型。
An experimental model of fetal growth restriction based on selective ligature of uteroplacental vessels in the pregnant rabbit.
机构信息
Department of Maternal-Fetal Medicine, Institut Clínic de Ginecologia, Obstetricia i Neonatologia, and Centro de Investigación Biomédica en Red de Enfermedades Raras, Barcelona, Spain. eixarch @ clinic.ub.es
出版信息
Fetal Diagn Ther. 2009;26(4):203-11. doi: 10.1159/000264063. Epub 2009 Dec 1.
INTRODUCTION
To describe an animal model of growth restriction based on selective ligature of uteroplacental vessels in the pregnant rabbit.
MATERIAL AND METHODS
Two experimental protocols (+21 and +25 days of gestation) with three groups were defined: controls, mild (20-30%) and severe (40-50%) uteroplacental vessel ligature. Fetuses were delivered 120 h after the procedure by cesarean section. Biometrical measurements were carried out. Brains were obtained and glial response and cell proliferation were studied by S100beta and Ki-67 immunohistochemistry.
RESULTS
Mortality rate and biometrical restriction increased across experimental groups according to the time and severity of the procedure. S100beta expression was significantly higher in the severe reduction group at 25 days. Ki-67 expression was significantly higher in the mild reduction group at 21 days and in the severe reduction group at 25 days.
DISCUSSION
Selective ligature of uteroplacental vessels in the pregnant rabbit results in a gradual model of growth restriction in terms of mortality, biometrical restriction and histological brain changes.
简介
描述一种基于选择性结扎妊娠兔的胎盘血管的生长受限动物模型。
材料与方法
确定了两个实验方案（妊娠第 21 天和第 25 天）和三个实验组：对照组、轻度（20-30%）和重度（40-50%）胎盘血管结扎组。手术后 120 小时通过剖宫产分娩胎儿。进行生物测量。通过 S100beta 和 Ki-67 免疫组化研究脑内神经胶质反应和细胞增殖。
结果
根据手术时间和严重程度，实验组的死亡率和生物测量受限均增加。在妊娠第 25 天，严重减少组的 S100beta 表达显著升高。在妊娠第 21 天和第 25 天，轻度减少组和严重减少组的 Ki-67 表达显著升高。
讨论
选择性结扎妊娠兔的胎盘血管可导致死亡率、生物测量受限和组织学脑改变方面逐渐出现生长受限模型。
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