真核生物中基因重组的生化分析。

Biochemical analysis of genetic recombination in eukaryotes.

作者信息

Pukkila P J

出版信息

Heredity (Edinb). 1977 Oct;39(2):193-217. doi: 10.1038/hdy.1977.61.

DOI:10.1038/hdy.1977.61

PMID:199560

Abstract

Recent studies concerning molecular mechanisms of genetic recombination in eukaryotes are reviewed. Since many of these studies have focused on the testable predictions arising from the hybrid DNA theory of genetic recombination, this theory is summarised. Experiments to determine the time of meiotic crossing-over and the structure of the synaptonemal complex which facilitates meiotic crossing-over are described. Investigations of DNA nicking and repair events implicated in recombination are discussed. Properties of proteins which may facilitate hybrid DNA formation, and biochemical evidence for hybrid DNA formation are presented. Finally, a nuclease which has been implicated in gene conversion is described.

摘要

本文综述了近期关于真核生物基因重组分子机制的研究。由于这些研究中有许多都聚焦于基因重组杂种DNA理论所产生的可检验预测，因此对该理论进行了总结。描述了确定减数分裂交叉发生时间以及促进减数分裂交叉的联会复合体结构的实验。讨论了与重组相关的DNA切口和修复事件的研究。介绍了可能促进杂种DNA形成的蛋白质特性以及杂种DNA形成的生化证据。最后，描述了一种与基因转换有关的核酸酶。