Sentenac Anne, Belkebir Kamal, Giovannini Hugues, Chaumet Patrick C
Institut Fresnel (UMR 6133), CNRS, Aix-Marseille Université, Campus de Saint Jérôme, 13013 Marseille, France.
J Opt Soc Am A Opt Image Sci Vis. 2009 Dec;26(12):2550-7. doi: 10.1364/JOSAA.26.002550.
We compare the performance of a total-internal-reflection fluorescence microscope under varying illumination and substrate conditions. The samples are deposited on a standard homogeneous glass slide or on a grating and illuminated by one or two interfering beams at various incident angles. A conjugate gradient with positivity a priori information is used to reconstruct the fluorophore density from the images. Numerical studies demonstrate that when the sample lies on an optimized grating, the lateral resolution of the microscope is greatly improved, up to fourfold, the best result being obtained when the grating is illuminated by two interfering beams.
我们比较了全内反射荧光显微镜在不同照明和底物条件下的性能。样品沉积在标准的均匀载玻片或光栅上，并由一束或两束干涉光束以不同入射角照射。使用具有先验正性信息的共轭梯度从图像中重建荧光团密度。数值研究表明，当样品置于优化的光栅上时，显微镜的横向分辨率可大幅提高，最高可达四倍，当光栅由两束干涉光束照射时可获得最佳结果。
