肾切除术后的肾癌发生。
Renal carcinogenesis after uninephrectomy.
机构信息
Department of Medicine and Therapeutics, The Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong SAR, China.
出版信息
Transl Oncol. 2009 Dec;2(4):258-63. doi: 10.1593/tlo.09142.
Nephrectomized rats have widely been used to study chronic renal failure. Interestingly, renal cell carcinoma occurred in the remnant kidney after uninephrectomy (UNX). In this study, we probed insulin-like growth factor (IGF)-1 signaling pathway in UNX-induced renal cancer. Adult male Sprague-Dawley rats were randomized into two groups: UNX rats (n = 22) and sham-operated rats (n = 12). Rats were killed at 3, 7, and 10 months. After 7 months after nephrectomy, the UNX rats developed renal cell carcinoma with increased expression of proliferating cell nuclear antigen, and 68.2% (15/22) of the animals exhibited invasive carcinoma. Western blot demonstrated significant down-regulation of IGF binding protein 3 contrasting with the up-regulation of protein kinase Czeta and Akt/protein kinase B in the renal cancer tissues. These findings indicate a unique rat model of UNX-induced renal cancer associated with enhanced IGF-1 signaling pathway.
肾切除大鼠被广泛用于研究慢性肾衰竭。有趣的是，单侧肾切除（UNX）后残余肾脏会发生肾细胞癌。在这项研究中，我们探讨了 UNX 诱导的肾癌中胰岛素样生长因子（IGF）-1 信号通路。成年雄性 Sprague-Dawley 大鼠随机分为两组：UNX 大鼠（n = 22）和假手术大鼠（n = 12）。大鼠分别在 3、7 和 10 个月时处死。肾切除后 7 个月，UNX 大鼠发生肾细胞癌，增殖细胞核抗原表达增加，68.2%（15/22）的动物发生浸润性癌。Western blot 显示 IGF 结合蛋白 3 显著下调，而蛋白激酶 Czeta 和 Akt/蛋白激酶 B 在肾癌组织中上调。这些发现表明存在一种与增强的 IGF-1 信号通路相关的独特 UNX 诱导的大鼠肾癌模型。
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